Objective To observe the effect of Wenjing Decoction （Decoction for warming meridians） on the expression of heme oxygenase 1（HO-1） and heme oxygenase 2（HO-2） in the ovary of model rats with blood stasis due to cold accumulation

and to explore its therapeutic mechanism in treating gynecological diseases.Methods Forty SD female rats were randomized into normal group

model group

and Wenjing Decoction low-dose and high-dose groups

10 in each.Wenjing Decoction low-dose and high-dose groups were administered Wenjing Decoction by gavage 14.66 or 29.32g/（kg·d）-1 respectively

once a day

for 2 weeks in succession.The other two groups were given the same volume distilled water.The plasma carbonic oxide（CO） activity was determined with the method of hemoglobin binding and dithionite reduction

the expression of HO-1 and HO-2 in ovarian granular and luteal cells was determined with the immunohistochemical SP method

and the expression of HO-1 mRNA and HO-2 mRNA in ovarian granular and luteal cells was determined with the in situ hybridization method.Results Compared with the model group

the activity of plasma COHb in the Wenjing Decoction low-dose and high-dose groups was significantly increased（P<0.05 or P<0.01）

while the expressions of HO-1

HO-2

and HO-1 mRNA and HO-2 mRNA in ovarian granular and luteal cells were dcreased（P<0.01 or P<0.05）.Conclusion The therapeutic effect of Wenjing Decoction on gynecological disease due to cold accumulation and blood stasis was related to regulating the expression of HO1 and HO-2.