Objective To observe the inhibition of the Zhisheng Capsule （To-life Capsule） in vivo and in vitro on human esophageal cancer Eca-109 cells and to study its functional mechanism. Methods In vivo:Fifty BALB/c mice were randomized into five groups

i.e. Zhisheng Capsule small

medium

large dosage group

cytoxan （CTX） group and blank control group

10 mice for each group. After medeling

Zhishen Capsule small

medium and large dosage group were given Zhisheng Capsule 0.25g/kg

0.5g/kg and 0.75g/kg respectively

CTX group was given CTX 40mg/kg

and blank control group was given normal saline. All groups were treated once per day for 10 days to observe changes of body weight and tumor weight. In vitro:Ten Japanese white rabbits were equally divided into samll

medium

and large dose of Zhisheng Capsule groups

CTX group

and blank control group. The medicine-contained serum was added into the Eca-109 cells suspension. After cultured for 72 hours

the optical density （OD） value was measured. The microscopic observation on the changes in morphology and number of cells were carried out. Results The effect of Zhisheng Capsule on tumor weight was stronger with the increase of concentration （P<0.05）

showing dosage-effect relation

body weight was increased accompained with the inhibition of tumor weight in Zhisheng Capsule medium dose group. The inhibition effect on Eca-109 cell was equal in Zhisheng Capsule large dose group and CTX group （P>0.05）. Zhisheng Capsule significantly inhibited Eca-109 cells with dose-effect rebation （P<0.05）. The microscopic observation showed that some cells became shrunken

changed to smaller in size and round in shape

decreased in refraction

and died of off-wall in all treated groups. Conclusion Zhisheng Capsule significantly inhibits human esophageal cancer Eca-109 cells with a dose-effect relationship. Its anti-tumor effect may be related to the cause of apoptosis of tumor cells.