Objective To explore the difference of toxic effect of Baifuzi （Rhizome Typhonii） on normal and kidney-yang insufficiency rats from the viewpoint of metabonomics.Methods Eighty SD male rats were randomized into normal group and kidney yang deficiency model group.After making the kidney yang deficiencyt model by injecting intramuscularly hydrocortisoni natrii succinas

the rats of two groups were randomized into the low

small

medium

large dose sub-groups and blank group with 8 in each.The medicated rats were administered by lavage the different concentrations of Rhizome Typhonii every day for 14 days

and the dosage was 0.32

0.64

1.28

2.56g/（kg·d）.The blank group was given normal saline. Twenty-four hours after the last medication

the blood plasma was obtained and used for testing adrenocorticotropic hormone （ACTH）

cortisol （COR）

cyclic adenosin monophosphate （cAMP） and cyclic guanosine monophosphate （cGMP） with the liquid chromatography-mass spectrometry （LC/MS） metabolomics technique.Results Hydrocortisone brought about the decline of lysophosphatidyl ethanolamine （LPE） and lysophosphatidyl choline （LPC） in blood plasma metabolite.There was a difference of endogenetic metabolite between the normal and model rats caused by white prepared lateral root of aconite.With the dose increase

a trend of decline of LPC and LPE of normal rats existed

however

those of the model rats kept basically stable.Conclusion The toxic reaction of the kidney-yang insufficiency rats to Rhizome Typhonii was weaker than that of the normal rats

confirmed the rationality of evaluating the toxicity of Chinese medicines based on TCM syndrome theory.