Objective To discuss the formation mechanism of blood-stasis syndrome in essential hypertension（EH）through the TLR-NF-κB signal transduction pathway and the effect of astragalus polysaccharide.Methods Eighty patients with EH were differentiated into blood-stasis syndrome group of 40 cases and non-blood-stasis syndrome group of 40 cases

and 30 healthy people were selected from volunteers（healthy group）.The serum of each subject of all groups was collected.The real-time PCR method was adopted to measure the expression of Toll-like receptor 4（TLR4） mRNA.Different concentrations of astragalus polysaccharide（APS）were given to intervene the expression of human umbilical venous endothelial cell clone TLR4 and the activation of nuclear transcription factors-κB（NF-κB）induced by lipopolysaccharide（LPS）.The PCR method was used to measure the expression of TLR4 and inhibitory κBα（I-κBa）mRNA and the immune imprinting method was applied to detect the expression of TLR4 protein and I-κBa protein.Results The expression of TLR4 mRNA in blood-stasis group and non-blood-stasis group was significantly higher than that of healthy group（P<0.01）

and it was significantly higher in the blood-stasis group than in the non-blood-stasis group（P<0.01）.The expression of TLR4 mRNA in the LPS induction group was significantly higher and the expression of I-κBa mRNA was significantly lower than that in the healthy group（P<0.01）.In the comparison of the APS big-

medium- and small-dose groups with the LPS induction group

the expressions of TLR4 and I-κBa mRNA were both statistically different（P<0.01）.And in the comparison among the APS big-

medium- and small-dose groups

the difference of expressions of TLR4 and I-κBa mRNA was also statistically significant（P<0.01）.Conclusion Hypertension patients with blood-stasis syndrome have more serious inflammation and immune disorder than those with non-blood-stasis syndrome.The inflammation and immune disorder mediated by TLR-NF-κB signal pathway is one of the mechanisms of formation of blood-stasis syndrome of EH.