Objective To discuss the effect and mechanism of Tuina manipulation for Gucuofeng （a joint derangement caused by violence or chronic strain and mainly manifested as pain and dysfunction of joints） of cervical spondylosis. Methods Twenty cases were randomized into Lijin （Regulating the tendons and muscles） group and Litiao （regulating the tendons and muscles and cervical vertebrae） group with 10 in each

and a normal group of 10 cases was established. The Litiao group was treated with manipulation on tendons and muscles and cervical vertebrae regulation

while the Lijin group and normal group were treated with manipulation on tendons and muscles. The treatment was given 3 times a week for 2 weeks. Before and after treatment

all patients were examined with Philip 64 spiral CT scanning from the base of skull to the first thoracic vertebra

the volume data was accessed

and the reconstructed thickness was 0.625mm. Then

the ITK-SANP software was used to fulfill the reconstruction of every segment of cervical vertebrae according to the CT data for comparing the changes of three-dimensional space of C2

C3

and C4 of the 3 groups after treatment. Results After treatment

the X

Y

and Z axes of C2 of the Litiao group were all significantly larger than those of the normal group （P<0.01）

and the X and Z axes were significantly larger than those of the Lijin group （P<0.05）. The Z axis of C2 of the Lijin group was significantly larger than that of the normal group （P<0.01）. The X and Y axes of C3 of the Litiao group were both significantly larger than those of the normal group （P<0.01）

and the X axis was significantly larger than that of the Lijin group （P<0.05）. The X axis of C3 of the Lijin group was significantly larger than that of the normal group （P<0.05）. The X and Z axes of C4 of the Litiao group were both significantly larger than those of the normal group and Lijin group （P<0.05 or P<0.01）. Conclusion Lijin manipulation plus Litiao manipulation on cervical vertebrae is effective in regulating the three-dimensional space location and improving the functions of cervical vertebrae. This is possibly one of its functional mechanisms for treating Gucuofeng of cervical spondylosis.