Objective To observe the effect of Zhonglun′e Decoction a Mongolia medicine

on the expression of anti-CⅡantibody

interleukin-1（IL-1）

interleukin-6（IL-6）and tumor necrosis factor-α（TNF-α）in rats with collagen induced arthritis （CIA）.Methods Eighty Wistar rats were randomized into control group（n=12）and model group（n=68）.CIA model was established through subcutaneous injection of typeⅡcollagen.Sixty selected models were randomized into six groups

i.e.the model group

Zhonglun′e Decoction small-dose group

Zhonglun′e Decoction middle-dose group

Zhonglun′e Decoction largedose group and tripterygium group

with 12in each.The Zhonglun′e Decoction small-dose group

middle-dose group and largedose group were given 0.35g/kg

0.7g/kg and 1.5g/kg of Zhonglun′e Decoction respectively.The tripterygium group was given 5mg/kg of tripterygium tablet.The control group and model group were given normal saline in the equal amount.The levels of anti-CⅡantibody

IL-1

IL-6and TNF-αwere detected by enzyme-linked immunosorbent assay（ELISA）after the administration through gastric feeding for 6weeks.Results Comparing with the control group

the levels of anti-CⅡantibody

IL-1

IL-6and TNF-αwas significantly increased in the model group（P<0.01）.The levels of anti-CⅡantibody and inflammatory cytokine were significantly decreased in the treatment groups.Both large-dose and middle-dose of Zhonglun′e Decoction as well as tripterygium tablet could decrease IL-1

IL-6and TNF-αwith similar effect.Conclusion Zhonglun′e Decoction could treat CIA by regulating immunity and decreasing the content of IL-1

IL-6and TNF-α.