Objective To investigate the effect of Shenqi Fuzheng Injection（Ginseng and Astragalus Right-Supporting Injection） on proliferation and cytotoxity of cytokine-induced killer（CIK） cells originated from umbilical cord blood.Methods CIK cells were induced and differentiated from the cord blood mononuclear cells and then were divided into Cytokine group

Shenqi Fuzheng Injection group

Cytokine plus Shenqi Fuzheng Injection group and the control group.Cytokine group was added γ-interferon（IFN-γ）

interleukin（IL）-1α

IL-2 and CD3.Shenqi Fuzheng Injection group was added Shenqi Fuzheng Injection.Cytokine plus Shenqi Fuzheng Injection group was added Shenqi Fuzheng Injection and IFN-γ

IL-1α

IL-2 and CD3.The control group was added only cluture solution.After 12 days of cultivation

the proliferation of CIK cells reached the peak.The phenotype and proliferative capacity of CIK cells was detected with flow cytometry

the cytotoxity was detected by MTT assay

and the level of IL-2

IFN-γ and tumor necrosis factor（TNF）-α was detected by ELISA assay.Results On the 12th day of cultivation

there was no significant difference in the proliferation of CIK cells between Cytokine group and Shenqi Fuzheng Injection group（P>0.05）

but with significant difference between Cytokine group and Cytokine plus Shenqi Fuzheng Injection group（P<0.01）.The killing effect of CIK cells against K562 cells in Cytokine group

Shenqi Fuzheng Injection group and Cytokine plus Shenqi Fuzheng Injection group was better than that of the control group（P<0.01）.CIK cells in Shenqi Fuzheng Injection group could secrete IL-2

IFN-γ and TNF-α.Conclusion Shenqi Fuzheng Injection could improve the proliferation and cytotoxicity of CIK cells and promote the secretion of IFN-γ

IL-2 and TNF-α as well.