Objective To observe the effect of fumigation lotion at different temperature on pain and function in knee osteoarthritis（KOA） patients and provide the basis for setting the optimum fumigation temperature.Methods Totally 126 KOA patients were randomized into three groups

with 42 in each.Three groups were given self-made fumigation lotion at 38℃

41℃ and 44℃ for 10 times respectively.The joint pain

function were observed before and after treatment by kujala score.Results There were significant differences in scores of joint pain and function after treatment in all the groups（P<0.05）.The fumigation lotion at 44℃ was the best effective in improving joint function as compared with the fumigation lotion at 38℃ and 41℃（P<0.05）.There was no significant difference in improving joint function between the fumigation lotion at 38℃ and 41℃（P>0.05）.The fumigation lotion at 41℃ was the best effective in relieving pain as compared with the fumigation lotion at 38℃ and 44℃（P<0.05）.The fumigation lotion at 38℃ was the least effective in relieving pain（P<0.05）.Conclusion The self-made fumigation lotion at 38℃

41℃ and 44℃ could improve the joint function and relieve pain.The fumigation lotion at 41℃ and 44℃ could be chosen according to the different situation of joint pain and function within tolerance of patients under safe condition.