Objective To observe the effect of Anfei Yishen Formula Lung-Quieting Kidney-Boosting Formula on pulmonary function and tissue injury in chronic obstructive pulmonary disease（COPD） rats.Methods Seventy-two healthy Wistar rats were randomized into Formula large

medium and small-dose groups

aminophylline group

model group and normal control group

with 12 in each.The COPD models were induced by intratracheal instillation of lipopolysaccharide and passive smoking.The rats in Formula large

medium and small-dose groups were intragastrically administered with Anfei Yishen Formula（crude drug 2.4g/kg

1.2g/kg and 0.6g/kg respectively）.The rats in aminophylline group were administered with 15mg/kg of aminophylline.The rats in model group and normal control group were administered with 0.5% of sodium carboxymethyl cellulose.The intragastric administration lasted for four weeks.Eight rats in each group were randomly selected to detect the pulmonary function such as forced expiratory volume in 0.2s（FEV0.2）

FEV0.2-to-forced vital capacity（FVC） ratio（FEV0.2/FVC

%）

expiratory resistance（Re） and inspiratory resistance（Ri）.The pathological changes in lung tissue of the rats were observed under HE staining.Results Comparing with the normal control group

FEV0.2 and FEV0.2/FVC significantly decreased and Re and Ri significantly increased in the model group（P<0.01）.Comparing with the model group

FEV0.2/FVC significantly increased and Re significantly decreased in Formula large

medium and small-dose groups（P<0.01）.FEV0.2 significantly increased in Formula large group（P<0.05）.FEV0.2 significantly increased and Re significantly decreased in Formula medium group（P<0.05 or P<0.01）.FEV0.2/FVC was higher and Re and Ri were lower in the Formula middle-dose group than in large and small dose group and the level of FEV0.2 was higher than that of the small-dose group.Anfei Yishen Formula could relieve the pathological inflammatory infiltration and reduce the formation of bullae in COPD rat lung tissue.Conclusion Anfei Yishen Formula could improve the pulmonary function and relieve the pathological changes of lung tissue in COPD rats.