Objective To observe the effects of kidney-supplementing therapy and liver-coursing therapy on the quantity and quality of oocytes in superovulated mice.Methods Ninety female Kunmin mice were randomized into control group

normal group

kidney-supplementing high-dose group

kidney-supplementing low-dose group

liver-coursing high-dose group and livercoursing low-dose group

with 15in each.The kidney-supplementing high-dose group and kidney-supplementing low-dose group was given 5.4g/ml and 2.7g/ml refined oral solution of kidney-supplementing menstruation-regulating formula respectively.The liver-coursing high-dose group and liver-coursing low-dose group was given 0.6g/ml and 0.3g/ml suspension of Xiaoyao Pill（Free Wanderer Pill）.The control group and normal group were given distilled water intragastrically.The mice in each group were gavaged with 1ml/100g for 10consecutive days.On the 11th day

mice in groups except the normal group were peritoneally injected gonadotropin from pregnant horse

and 48hours afterwards

human chorionic gonadotropin was injected peritoneally.The serum estradiol（E2）

luteinizing hormone（LH）and follicle-stimulating hormone（FSH）was detected after 16hours.The ovulation rates and quality follicle counts were compared.The expressions of growth and differentiation factor-9（GDF9）protein and GDF9mRNA in oocytes were detected.Results Comparing with the control group

the expressions of FSH

LH

E2

GDF9and GDF9mRNA were significantly increased in other groups（P<0.05）

especially in two high-dose administration groups as comparing with two low-dose administration groups（P<0.05）.The quality follicle counts in the administration groups were significantly higher than that in the control group（P<0.05）.Comparing with the normal group

the ovulation rates in the control group and administration groups were significantly increased（P<0.05）.Conclusion The kidney-supplementing therapy and liver-coursing therapy can increase the quantity of oocytes

improve the rate of quality follicular and promote ovum

which may be relevant to regulating the expression of GDF9in oocytes.