Objective To research the effect and mechanism of ear-body acupoint electro-acupuncture（EA）on the bile secretion in rats.Methods Totally 42Wistar rats were randomized into five groups.The point EA group（n=10）was given EA on the liver and gallbladder area（CO11and CO12）of the ear and on"Danshu"（BL19）.The non-point EA group（n=8）was given EA on the helix and 1.0cm next to Danshu.The No.1injection group（n=8）was given tail vein injection of 2ml/kg duodenal extract of rats in the point EA group.The No.2injection group（n=8）was given tail vein injection of 2ml/kg duodenal extract of rats in the non-point EA group.The normal saline injection group（n=8）was given tail vein injection of 2ml/kg normal saline.Each group was given intubation of common bile duct and the bile flow was observed at different time points.Results The bile flow was significantly increased in the point EA group in the 10th

30th and 50th minute of EA and 10

30

50

70

90and 110minutes after EA（P<0.05or P<0.01）.The bile flow in the point EA group was significantly higher than that in the non-point EA group at each time points（P<0.05or P<0.01）.The bile flow in the No.1injection group was significantly increased 10

30

50

70

90and 110minutes after EA（P<0.05or P<0.01）.The bile flow in the No.2injection group was significantly lower than that in the No.1injection group 10or 50minutes after EA（P<0.05or P<0.01）.There was no significant difference between the point EA group and No.1injection group within 10minutes after EA or injection（P>0.05）.Conclusion The ear-body point EA can increase the bile flow with a better after-action.Its mechanism may be relevant to adjusting the biliary system through humoral regulation.