Objective To observe the antibacterial activity of Xiaodu Yuji Solution （Skin Healing Disinfectant Solution） on diabetic foot ulcers in rats.Methods Sixty rats were selected and the diabetic foot models were established with intraperitoneal injection of streptozotocin and thin sheet iron fixation after 4 weeks of high-fat high-protein feeding.The bacterial culture and separation was conducted on wound secretions.Xiaodu Yuji Solution （concentration 1 g/ml）

piperacillin （concentration 500 μg/ml）

levofloxacin （concentration 500 μg/ml）

fluconazole （concentration 300 μg/ml） and normal saline were used for in vitro antibacterial test.The effects of various drugs on the in vitro inhibition zone diameters of different pathogens were compared.Results Eighty-six pathogens were cultured in 60 secretion samples.There were 41 （47.7%） gram-positive bacteria

37 gram-negative bacteria （43.0%） and 8 （9.3%） fungi.Xiaodu Yuji Solution had high sensitivity to gram-negative bacteria

gram-positive bacteria and fungi.The sensitivity of Xiaodu Yuji Solution to Staphylococcus aureus

Staphylococcus epidermidis and Streptococcus was superior to that of levofloxacin （P<0.05 or P<0.01）.The sensitivity of Xiaodu Yuji Solution to Escherichia coli

Enterobacter cloacae and Proteus bacteria was superior to that of piperacillin （P<0.01 or P<0.05）.The sensitivity of Xiaodu Yuji Solution to Candida tropicalis was superior to that of fluconazole （P<0.05）.Conclusion Xiaodu Yuji Solution has obvious antimicrobial effect on diabetic foot ulcers.