Objective To explore the correlation between bioelectricity information value of twelve Yuan-Source acupoints and the total score of Subjective Fatigue Scale（SFS）in the balanced constitution population and qi-deficiency constitution population.Methods Twenty-nine cases of qi-deficiency constitution and 29cases of balanced constitution were selected according to Chinese Medical Constitution Questionnaire.The degree of subjective fatigue was evaluated with SFS.The bioelectricity information vaule of bilateral twelve Yuan-Source acupoints i.e.Taiyuan（LU 9）

Daling（PC 7）

Shenmen（HT 7）

Wangu（SI 4）

Yangchi（SJ 4）

Hegu（LI 4）

Taibai（SP 3）

Taichong（LR 3）

Taixi（KI 3）

Jinggu（BI 64）

Qiuxu（GB 40）and Chongyang（ST 42）were detected with system status electronic measuring instrument.The correlation between twelve Yuan-Source acupoint bioelectricity information value and the total score of SFS was analyzed.Results The total score of SFS in the qi-deficiency constitution population was（82.24±19.60）which was significantly higher than（58.24±11.71）in the balanced constitution population（P <0.01）.There was significant difference between groups in the bioelectricity information value of the right Hegu（LI 4）（P<0.05）

but with no significant difference in the bioelectricity information values of other acupoints（P>0.05）.The total score of SFS was significantly correlated with the bioelectricity information values of left Daling（PC 7）

left Taichong（LR 3）

right Hegu（LI 4）and right Qiuxu（GB 40）（P<0.05）in the total 58cases.In the qi-deficiency constitution population

the total score of SFS was negatively correlated with the bioelectricity information values of left Daling（PC 7）and right Taichong（LR 3）（P<0.05）.In the balanced constitution population

the total score of SFS was positively correlated with the bioelectricity information value of left Taiyuan（LU 9）（P<0.05）.Conclusion The bioelectricity information value of twelve Yuan-Source acupoints was correlated with the total score of SFS in the balanced constitution population and qi-deficiency constitution population.The detection of Yuan-Source acupoint bioelectricity information value can provide reference for fatigue detection.