Objective To study the effect of moxibustion on gastric mucosal injury repair related cell signaling proteins and its molecular mechanism.Methods Forty SD rats were randomized into the normal（blank）group

model group

stomach channel point group and control point group

with 10in each.The stress ulcer rat models were established with restraint cold stress method.The stomach channel point group was given moxibustion on Liangmen（ST21）and Zusanli（ST36）.The control point group was given moxibustion on the control points.The rats in the blank and model groups were tied with mouse board only.Each group was given corresponding measures 20min each time and once daily for 12days.The mucosal cell protein fingerprint was detected with protein fingerprinting

hydrophobic chip

hydrophilic chip and metal ion chelating chip.The mass-to-charge ratio of differential protein in each group was compared and analyzed. Results Comparison of protein fingerprints in each group showed that 10signs of differentially expressed proteins were detected in 3chips.Three signs of differentially expressed proteins were detected in hydrophobic chip and their molecular weights were 2736Da

6985Da and7324Da.One sign of differentially expressed protein was detected in hydrophilic chip and its molecular weight was 4561Da.Six signs of differentially expressed proteins were detected in metal ion chelating chip and their molecular weights were 1034Da

2136Da

3261Da

4871Da

7586Da and 8267Da.Conclusion Moxibustion can regulate the differential expression in a variety of related signaling proteins and promote the repairation of gastric mucosal injury.