Objective To observe the effect of Wuzi Sanhuang Formula [formula of Ziheche（ Placenta Hominis

紫河车）

Zidanshen（ Radix et Rhizoma Salviae Miltiorrhizae

紫丹参）

Ziyuan（ Radix et Rhizoma Asteris

紫苑）

Zisuzi（ Fructus Perillae

紫苏子）

Zishiying（ Fluoritum

紫石英）

Huangjing（ Rhizoma Polygonati

黄精）

Huangqin（ Radix Scutellariae

黄芩） and Mahuang（ Herba Ephedrae

麻黄） ] on the function of hypothalamicpituitary-adrenal（ HPA） axis in asthmatic mice intervened with prednisone. Methods Totally 120 BALB / C mice were randomized into the blank group

model group

Prednisone Acetate Tablets group and Wuzi Sanhuang Formula low-dose

middle-dose and high-dose groups

with 20 in each. The blank group was given intraperitoneal injection and atomization of normal saline. The other five groups were given intraperitoneal injection of 10 μg ovalbumin and 2 mg aluminum hydroxide gel on the 1st

7th and 14th day and 1% ovalbumin atomization from the 21st day once daily for 7 consecutive days and once every three days with a total of 18 times. The Prednisone Acetate Tablets group and Wuzi Sanhuang Formula low-dose

middle-dose and high-dose groups were gavaged with 3. 9 mg / kg prednisone acetate before each atomization. The steroid was withdrawn from the 42nd day at a dose of 0. 325 mg / kg every other day and completely withdrawn on the 63rd day. The Wuzi Sanhuang Formula low-dose

middle-dose and high-dose groups were gavaged with 1. 994 g / kg

3. 988 g / kg and 7. 976 g / kg Wuzi Sanhuang Formula granules and the model group and blank group were gavaged with the same amount of normal saline from the 35th day. The contents of corticotropin releasing hormone（ CRH）

adrenocorticotropic hormone（ ACTH） and corticosterone（ COR） were detected with radioimmunoassay on the 64th day. Results There was no signficant difference between the model group and blank group in the contents of COR

CRH and ACTH（ P > 0. 05）. Comparing with the model group

the indicators in all administration groups were significantly decreased（ P < 0. 05 or P < 0. 01）. Comparing with the Prednisone Acetate Tablets group

the indicators in the Wuzi Sanhuang Formula groups were significantly increased（ P < 0. 05 or P < 0. 01）

especially in the Wuzi Sanhuang Formula high-dose group（ P < 0. 05 or P < 0. 01）. Conclusion Wuzi Sanhuang Formula can improve the function of HPA axis in asthmatic mice intervened with prednisone

especially high dose.