Objective To study the possible mechanism of Gandouling [tablets for Wilson’s disease（ WD） ]for WD-induced liver fibrosis.Methods Totally 75 Wistar rats were randomized into the blank control group

model control group

Gandouling group

Gandouling combined with Penicillamine group

and Penicillamine group

with 15in each.The copper-loaded rat models were copied for 12 weeks in all groups except the blank control group.The Gandouling group was gavaged with 0.486 g /（ kg·d） of Gandouling

the Penicillamine group was gavaged with 0.09g /（ kg · d） of Penicillamine

and the Gandouling combined with Penicillamine group was gavaged with 0.486 g /（ kg·d） of Gandouling and 0.09g/（ kg·d） Penicillamine once daily from the 7th week to the 13th week.The serum specimens were taken and the small molecule makers of liver fibrosis were detected with metabolomic techniques.Results Gandouling could increase the contents of leucine

isoleucine

choline

glucose and 3-hydroxybutyric acid

and decrease the contents of glycoproteins

lipids

lactate and pyruvate in copper-loaded rats.Conclusion Gandouling can regulate small molecule metabolites in copper-loaded rats and repair metabolic abnormalities

which maybe one of the mechanisms for WD.