Objective To study the possible mechanism of Huiyang Shengji Powder（ powder to return yang and engender flesh

回阳生肌散） on angiogenesis-related factors in rat with diabetic skin ulcer. Methods Fifty-six SD rats were randomized into the control group（ n = 8）

model group（ n = 12）

whole formula group（ n = 12）

Wenyang group（ n = 12） and Huoxue group（ n = 12）. The rat models of diabetic skin ulcer were established by intraperitoneal injection of streptozotocin（ STZ）

hormone intervention

artificial skin defects and stimulation of plastic ring in all groups except the control group. The control group and model group were given petrolatum gauze. Huiyang Shengji Powder was disassembled into Wenyang Yiqi Formula（ Yang-Warming Qi-Boosting Formula

温阳益气方） and Huoxue Shengji Formula（ Blood-Quickening Flesh-Engendering Formula

活血生肌方）. The whole formula group

Wenyang group and Huoxue group were given ointment gauze with Huiyang Shengji Powder

Wenyang Yiqi Formula and Huoxue Shengji Formula respectively. Each group was treated once daily for 15 days. The expressions of vascular endothelial growth factor（ VEGF）

vascular endothelial growth factor receptor-2（ VEGFR-2） and endothelial nitric oxide synthase（ eNOS） in granulation tissue were detected. Results The expression levels of VEGF

VEGFR-2 and eNOS in the model group were significantly lower than those in the control group（ P < 0. 01）. The expression level of VEGF in the whole formula group was significantly higher than that in the Huoxue group and model group（ P < 0. 01）. The expression levels of VEGFR-2 and eNOS in the whole formula group were significantly higher than those in the Wenyang group

Huoxue group and model group（ P < 0. 01）. Conclusion Huiyang Shengji Powder can promote wound healing by increasing the expression levels of VEGF

VEGFR-2 and eNOS in granulation tissue.