Objective To research the effects of acupoint application of Mustard Seeds with different proportions on the immune functions in patients with bronchial asthma. Methods Totally 90 cases of bronchial asthma were randomized into the raw Mustard Seeds group

half raw and half fried Mustard Seeds group and fried Mustard Seeds group

with 30 in each. In the raw Mustard Seeds group

raw Mustard Seeds

Gansui（ Radix Kansui）

Yanhusuo（ Rhizoma Corydalis） and Xixin（ Radix et Rhizoma Asari） were used at a ratio of 2 ∶ 2 ∶ 1 ∶ 1. In the half raw and half fried Mustard Seeds group

raw Mustard Seeds

fried Mustard Seeds

Gansui

Yanhusuo and Xixin were used at a ratio of 1 ∶ 1 ∶ 2 ∶ 1 ∶ 1. In the fried Mustard Seeds group

fried Mustard Seeds

Gansui

Yanhusuo and Xixin were used at a ratio of 2 ∶ 2 ∶ 1 ∶ 1. In each group

the acupoint application was conducted on Tianshu（ CV22）

Danzhong（ CV17）

Dingchuan（ EX-B1）

Fengmen（ BL12） and Feishu（ BL13） in the dog days（ three periods forming the hottest periods of summer） once each period for three times. The efficacy

skin reactions

immunoglobullins and eosinophils（ EOS） were observed after treatment. Results The cured

marked effective

effective and ineffective cases in the raw Mustard Seeds group were 3

14

11 and 2 respectively

while there were 1 cured

14 marked effective

12 effective and 3 ineffective in the half raw and half fried Mustard Seeds group

and there were 1 cured

10 marked effective

11 effective and 8 ineffective in the fried Mustard Seeds group

with no significant difference among groups（ P > 0. 05）. The total scores of skin reactions in the three groups

were significantly decreased after treatment（ P < 0. 05）. Differences in the inimmunoglobulins and EOS between before and after treatment were significant in all groups（ P < 0. 05 or P < 0. 01）. The serum IgG level in the fried Mustard Seeds group was significantly lower than that in the raw Mustard Seeds group（ P < 0. 05）. Conclusion The acupoint application of Mustard Seeds with different proportions can effectively relieve symptoms and improve immune functions in patients with bronchial asthma. The skin reactions are gradually increased as increasing the proportion of raw Mustard Seeds

but the degree of skin reaction has no significant effect on immune function.