Objective To research the effects of Yiqi Huoxue Formula（qi-supplementing blood-quickening formula

益气活血方）contained serum with different concentrations on the proliferation of bone marrow mesenchymal stem cells（BMMSCs）and the expression of vascular endothelial growth factor（VEGF）in rats. Methods Eight SD rats were randomized into blank control group and Yiqi Huoxue Formula group with 4rats in each group.The BMMSCs were purified and cultured in rats.The serums were collected in the blank control group（intragastric administration with distilled water）and Yiqi Huoxue Formula group（intragastric administration with Yiqi Huoxue Formula）.The BMMSCs were divided into the blank serum control group（BMMSCs were cultured by 10%blank serum）and 5%

10%and 20%Yiqi Huoxue Formula serum groups（BMMSCs were cultured by 5%

10% and 20%Yiqi Huoxue Formula serums respectively）.The proliferation activity of BMMSCs was measured by MTT assay.The expression of VEGF in cultured BMMSCs supernatant was detected by ELISA assay. Results Comparing with the 5% and 20% Yiqi Huoxue Formula serum groups

the baseline removal proliferation rate of BMMSCs in the 10% Yiqi Huoxue Formula serum group was significantly increased on the 2nd

4th and 6th day（P<0.05or P<0.01）.Comparing with the blank serum control group and 5%and 20% Yiqi Huoxue Formula serum groups

the levels of VEGF in the 10% Yiqi Huoxue Formula serum group were significantly increased on the 2nd and 4th day（P<0.01）. Conclusion Yiqi Huoxue formula contained serum at 10percent can significantly improve the proliferation of BMMSCs and promote the expression of VEGF.