大黄丹参水煎剂对胰腺纤维化大鼠转化生长因子β<sub>1</sub>、血小板衍生生长因子BB及其mRNA表达的影响

梁晓强; 王玉凤; 章学林; 顾宏刚; 马恩伟; 张静喆

doi:10.13288/j.11-2166/r.2014.15.019

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大黄丹参水煎剂对胰腺纤维化大鼠转化生长因子β₁、血小板衍生生长因子BB及其mRNA表达的影响

实验研究 | 更新时间：2021-11-17

- 大黄丹参水煎剂对胰腺纤维化大鼠转化生长因子β₁、血小板衍生生长因子BB及其mRNA表达的影响
- Effects of Dahuang Danshen Decoction on the Expressions of TGF-β₁,PDGF-BB,TGF-β₁ mRNA and PDGF-BB mRNA in Rats With Pancreatic Fibrosis
- 中医杂志 2014年55卷第15期页码：1331-1334
- 作者机构：
  
  上海中医药大学附属龙华医院
- 作者简介：
- 基金信息：
  
  国家自然科学基金资助项目（81173267）;上海市卫生局海派中医流派传承研究基地建设资助项目（ZYSNXD-CC-APGC-JD002）;上海市教委预算内科研资助项目（2010JW36）
- DOI：10.13288/j.11-2166/r.2014.15.019
  中图分类号： R285.5
- 纸质出版：2014
- 稿件说明：
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[1]梁晓强,王玉凤,章学林,顾宏刚,马恩伟,张静喆.大黄丹参水煎剂对胰腺纤维化大鼠转化生长因子β_1、血小板衍生生长因子BB及其mRNA表达的影响[J].中医杂志,2014,55(15):1331-1334.

LIANG Xiaoqiang, WANG Yufeng, ZHANG Xuelin, et al. Effects of Dahuang Danshen Decoction on the Expressions of TGF-β₁,PDGF-BB,TGF-β₁ mRNA and PDGF-BB mRNA in Rats With Pancreatic Fibrosis[J]. Journal of Traditional Chinese Medicine2014, 55(15): 1331-1334. DOI: 10.13288/j.11-2166/r.2014.15.019.
[1]梁晓强,王玉凤,章学林,顾宏刚,马恩伟,张静喆.大黄丹参水煎剂对胰腺纤维化大鼠转化生长因子β_1、血小板衍生生长因子BB及其mRNA表达的影响[J].中医杂志,2014,55(15):1331-1334. DOI： 10.13288/j.11-2166/r.2014.15.019.

LIANG Xiaoqiang, WANG Yufeng, ZHANG Xuelin, et al. Effects of Dahuang Danshen Decoction on the Expressions of TGF-β₁,PDGF-BB,TGF-β₁ mRNA and PDGF-BB mRNA in Rats With Pancreatic Fibrosis[J]. Journal of Traditional Chinese Medicine2014, 55(15): 1331-1334. DOI: 10.13288/j.11-2166/r.2014.15.019. DOI：

摘要

目的观察大黄丹参水煎剂抗胰腺纤维化的可能作用机制。方法 50只雄性Wistar大鼠随机分为正常组、模型组和大黄丹参水煎剂低、中、高剂量组

每组10只。腹腔注射二乙基二硫代氨基甲酸钠（DDC）6周建立胰腺纤维化模型。大黄丹参水煎剂低、中、高剂量组在造模同时分别给予大黄丹参水煎剂0.137、0.274、0.548 g/（kg·d）灌胃

正常组和模型组给予等量生理盐水灌胃

连续给药6周。免疫组化检测胰腺α-平滑肌肌动蛋白（α-SMA）表达

RT-PCR检测胰腺大鼠转化生长因子β1（TGF-β1）mRNA、血小板衍生生长因子BB（PDGF-BB）mRNA表达

免疫印迹法检测TGF-β1蛋白、PDGF-BB蛋白表达。结果正常组α-SMA低表达

模型组α-SMA高表达

大黄丹参水煎液各剂量组α-SMA均有不同程度的减弱。模型组TGF-β1、PDGF-BB蛋白B mRNA表达较正常组上调（P<0.05或P<0.01）;与模型组比较

大黄丹参中、高剂量组TGF-β1蛋白及mRNA表达下调

大黄丹参高剂量组PDGF-BB蛋白及mRNA下调（P<0.05或P<0.01）。与大黄丹参中剂量组比较

大黄丹参高剂量组TGF-β1蛋白下降显著（P<0.05）。结论"下调TGF-β1及PDGF-BB及其mRNA表达—抑制α-SMA表达—抑制PSC活化—减少ECM合成—改善ECM沉积"可能是大黄丹参抗胰腺纤维化的作用机制之一。

Abstract

Objective To observe the effect and mechanism of Decoction of Dahuang（Rhei Radix et Rhizoma）-Danshen（Salviae Miltiorrhizae Radix） on rats with pancreatic fibrosis. Methods Fifty male Wistar rats were randomized into the normal group

model group and high-dose

middle-dose and low-dose Dahuang-Danshen groups

with 10 in each. The pancreatic fibrosis models were established by intraperitoneal injection of diethyl dithiocarbamate（DDC） for 6 weeks. The low-dose

middle-dose and high-dose Dahuang-Danshen groups were gavaged with 0.137g/（kg·d）

0.274g/（kg·d） and 0.548g/（kg·d） Dahuang-Danshen respectively. The normal group and model group were gavaged with same volume of normal saline. The administration lasted for 6 weeks. The expressions of α-SMA

transforming growth factor-β1（TGF-β1） TGF-β1 mRNA

platelet-derived growth factor-BB（PDGF-BB） and PDGF-BB mRNA in pancreas were detected with immunohistochemical detection

RT-PCR and Western Blotting respectively. Results The expression of α-SMA was low in the normal group but high in the model group. The expression of α-SMA was decreased in the Dahuang-Danshen groups. Comparing with the normal group

the expressions of TGF-β1 mRNA and PDGF-BB mRNA were significantly increased in the model group（P < 0. 05）. Comparing with the model group

the expression of TGF-β1 mRNA was significantly decreased in middle-dose and high-dose Dahuang-Danshen groups and the expression of PDGF-BB mRNA was significantly decreased in high-dose DahuangDanshen group（ P < 0. 05）. Comparing with middle-dose Dahuang-Danshen group

the expression of TGF-β1 was significantly decreased in high-dose Dahuang-Danshen group（P < 0. 05）. Conclusion The anti-pancreatic fibrosis mainline of Dahuang-Danshen may be down-regulating the expressions of TGF-β1

TGF-β1 mRNA

PDGF and PDGF mRNA

inhibiting the expression of α-SMA

inhibiting the activation of PSC

reducing the synthesis of ECM and improving the deposition of ECM.

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大黄丹参水煎剂对胰腺纤维化大鼠转化生长因子β1、血小板衍生生长因子BB及其mRNA表达的影响

Effects of Dahuang Danshen Decoction on the Expressions of TGF-β1,PDGF-BB,TGF-β1 mRNA and PDGF-BB mRNA in Rats With Pancreatic Fibrosis

DOI：10.13288/j.11-2166/r.2014.15.019

摘要

Abstract

关键词

Keywords

references

大黄丹参水煎剂对胰腺纤维化大鼠转化生长因子β₁、血小板衍生生长因子BB及其mRNA表达的影响

Effects of Dahuang Danshen Decoction on the Expressions of TGF-β₁,PDGF-BB,TGF-β₁ mRNA and PDGF-BB mRNA in Rats With Pancreatic Fibrosis