清热燥湿凉血方对溃疡性结肠炎大鼠结肠组织修复的影响

张夏梦; 寿折星; 范恒; 石月萍

doi:10.13288/j.11-2166/r.2014.20.015

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清热燥湿凉血方对溃疡性结肠炎大鼠结肠组织修复的影响

实验研究 | 更新时间：2021-11-17

- 清热燥湿凉血方对溃疡性结肠炎大鼠结肠组织修复的影响
- Effects of Heat-Clearing Dampness-Drying Blood-Cooling Formula on the Repair in Colon Tissue of Rats with Ulcerative Colitis
- 中医杂志 2014年55卷第20期页码：1764-1767
- 作者机构：
  
  1. 宜昌市第二人民医院
  2. 华中科技大学同济医学院附属协和医院
  3. 辽宁医学院附属第一医院
- 作者简介：
- 基金信息：
  
  国家自然科学基金资助项目（81102690）
- DOI：10.13288/j.11-2166/r.2014.20.015
  中图分类号： R285.5
- 纸质出版：2014
- 稿件说明：
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[1]张夏梦,寿折星,范恒,石月萍.清热燥湿凉血方对溃疡性结肠炎大鼠结肠组织修复的影响[J].中医杂志,2014,55(20):1764-1767.

ZHANG Xiameng, SHOU Zhexing, FAN Heng, et al. Effects of Heat-Clearing Dampness-Drying Blood-Cooling Formula on the Repair in Colon Tissue of Rats with Ulcerative Colitis[J]. Journal of Traditional Chinese Medicine2014, 55(20): 1764-1767.
[1]张夏梦,寿折星,范恒,石月萍.清热燥湿凉血方对溃疡性结肠炎大鼠结肠组织修复的影响[J].中医杂志,2014,55(20):1764-1767. DOI： 10.13288/j.11-2166/r.2014.20.015.

ZHANG Xiameng, SHOU Zhexing, FAN Heng, et al. Effects of Heat-Clearing Dampness-Drying Blood-Cooling Formula on the Repair in Colon Tissue of Rats with Ulcerative Colitis[J]. Journal of Traditional Chinese Medicine2014, 55(20): 1764-1767. DOI： 10.13288/j.11-2166/r.2014.20.015.

摘要

目的探讨清热燥湿凉血方治疗溃疡性结肠炎（UC）的可能作用机制。方法将40只SD大鼠随机分为正常组、模型组、艾迪莎组、中药组

每组10只。模型组、艾迪莎组、中药组用三硝基苯磺酸/乙醇复合法局部灌肠建立UC大鼠模型

24h后正常组和模型组以生理盐水1ml/100g灌肠

艾迪莎组以艾迪莎混悬液（浓度为5g/L）1ml/100g灌肠

中药组予以清热燥湿凉血方混悬液（含生药1g/ml）1ml/100g灌肠

每天1次

各组均灌肠15天。取结肠组织进行病理学观察

免疫荧光染色检测结肠组织CK20、CD34蛋白的表达

RT-PCR技术检测结肠组织CK20、CD34、核因子-κB（NF-κB）mRNA的表达。结果模型组、艾迪莎组、中药组CK20、CD34蛋白及mRNA的表达均明显高于正常组（P<0.01）

艾迪莎组和中药组CK20、CD34蛋白及mRNA表达均明显高于模型组（P<0.01）;艾迪莎组、中药组NF-κB mRNA表达明显低于模型组（P<0.01）。结论清热燥湿凉血方能促进UC大鼠结肠组织上皮修复和结肠微血管生成

抑制结肠组织NF-κB的表达

可能是治疗UC的作用机制之一。

Abstract

Objective To study the possible mechanism of Heat-Clearing Dampness-Drying Blood-Cooling Formula（ HDBF） for ulcerative colitis（ UC）. Methods Forty SD rats were randomized into the normal group

model group

Etiasa group and Chinese medicine group

with 10 in each. The rat models of UC were established in the model group

Etiasa group and Chinese medicine group. The normal group and model group were lavaged with normal saline once daily 24 hours later. The Etiasa group was lavaged with Etiasa and Chinese medicine group was lavaged with HDBF once daily 24 hours later. The lavation lasted for 15 days. The colon tissue specimens were observed. The expressions of CK20

CK20 mRNA

CD34

CD34 mRNA and nuclear factor-κB（ NF-κB） mRNA were detected. Results The expressions of CK20

CK20 mRNA

CD34 and CD34 mRNA in the model group

Etiasa group and Chinese medicine group were significantly higher than those in the normal group（ P < 0. 01）. The expressions of CK20

CK20 mRNA

CD34 and CD34 mRNA in the Etiasa group and Chinese medicine group were significantly higher than those in the model group（ P < 0. 01）. The expression of NF-κB mRNA in the Etiasa group and Chinese medicine group was significantly lower than that in the model group（ P < 0. 01）. Conclusion HDBF can promote epithelial repair and angiogenesis in colon tissue of UC rats and inhibit the expression of NF-κB

which may be one mechanism in treating UC.

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