通络救脑注射液及其单体成分对缺氧诱导损伤神经元的影响

王筠; 侯金才; 向丽华; 鞠大宏

doi:10.13288/j.11-2166/r.2014.21.016

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通络救脑注射液及其单体成分对缺氧诱导损伤神经元的影响

实验研究 | 更新时间：2021-11-17

- 通络救脑注射液及其单体成分对缺氧诱导损伤神经元的影响
- Effects of Tongluo Jiunao Injection and Its Compositions on Neuron Damage Induced by Anoxia
- 中医杂志 2014年55卷第21期页码：1860-1863
- 作者机构：
  
  1. 中国中医科学院中医基础理论研究所
  2. 中国中医科学院西苑医院
- 作者简介：
- 基金信息：
  
  国家自然科学基金资助项目（81102679）;中国中医科学院基本科研业务费自主选题项目（ZZ070824）
- DOI：10.13288/j.11-2166/r.2014.21.016
  中图分类号： R285
- 纸质出版：2014
- 稿件说明：
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[1]王筠,侯金才,向丽华,鞠大宏.通络救脑注射液及其单体成分对缺氧诱导损伤神经元的影响[J].中医杂志,2014,55(21):1860-1863.

WANG Jun, HOU Jincai, XIANG Lihua, et al. Effects of Tongluo Jiunao Injection and Its Compositions on Neuron Damage Induced by Anoxia[J]. Journal of Traditional Chinese Medicine2014, 55(21): 1860-1863.
[1]王筠,侯金才,向丽华,鞠大宏.通络救脑注射液及其单体成分对缺氧诱导损伤神经元的影响[J].中医杂志,2014,55(21):1860-1863. DOI： 10.13288/j.11-2166/r.2014.21.016.

WANG Jun, HOU Jincai, XIANG Lihua, et al. Effects of Tongluo Jiunao Injection and Its Compositions on Neuron Damage Induced by Anoxia[J]. Journal of Traditional Chinese Medicine2014, 55(21): 1860-1863. DOI： 10.13288/j.11-2166/r.2014.21.016.

摘要

目的探讨通络救脑注射液保护神经元的可能作用机制。方法制备小胶质细胞氧糖剥夺条件液、通络救脑条件液、栀子苷条件液、人参皂苷Rg1条件液

将神经元分为正常对照组、氧糖剥夺组、正常条件液组、氧糖剥夺条件液组、通络救脑条件液组、栀子苷条件液组、人参皂苷Rg1条件液组

其中正常对照组采用常规神经元培养液

氧糖剥夺组采用无糖DMEM培养液

其余5组按照处理条件分别使用对应的小胶质细胞条件培养液

条件培养液添加为100%

作用时长为6h。比较各组神经元细胞活性、乳酸脱氢酶（LDH）漏出率、神经元NMDAR1及caspase-3的表达水平。结果与正常对照组比较

氧糖剥夺组和氧糖剥夺条件液组神经元细胞活性明显下降

LDH漏出率明显增加

神经元NMDAR1和caspase-3表达均明显上升（P<0.05或P<0.01）。与氧糖剥夺组比较

通络救脑条件液组、栀子苷条件液组、人参皂苷Rg1条件液组神经元细胞活性明显提高

通络救脑条件液组和栀子苷条件液组LDH漏出率降低、NMDAR1表达明显下降（P<0.05或P<0.01）

通络救脑条件液组、人参皂苷Rg1条件液组caspase-3表达明显下降（P<0.05或P<0.01）。结论通络救脑注射液及其单体成分（栀子、三七有效组分）可通过调节小胶质细胞的分泌成分

降低LDH漏出率及神经元NMDAR1、caspase-3的表达水平

提高神经元活性

逆转损伤小胶质细胞可能对神经元造成的影响

从而发挥神经元的保护作用

并且通络救脑注射液有整合调节的治疗优势。

Abstract

Objective To investigate the possible action mechanism of Tongluo Jiunao Injection in protecting neurons. Methods The microglia glucose deprivation（ OGD） conditioned medium

Tongluo Jiunao conditioned medium

geniposide conditioned medium and ginsenoside-Rg1 conditioned medium were prepared. The neurons were classified into the normal control group

OGD group

normal conditioned medium group

OGD conditioned medium group

Tongluo Jiunao conditioned medium group

geniposide conditioned medium group and ginsenoside-Rg1 conditioned medium group. In normal control group

neurons were cultured in conventional way

OGD group was treated with glucose-free DMEM culture and the rest 5 groups were cultured in the responding microglia conditioned mediums.The conditioned mediums were added as 100% and the action time was 6 hours. The cell viability

leakage rate of lactate dehydrogenase（ LDH） and expressions of NMDAR1 and caspase-3 of neurons in each group were compared.Results Comparing with the normal control group

the neuron viability was significantly decreased

LDH leakage rate was significantly increased and expressions of neuron NMDAR1 and caspase-3 were significantly increased in OGD group and OGD conditioned medium group（P<0.05 or P<0.01）. Comparing with the OGD group

the neuron viability in Tongluo Jiunao conditioned medium group

geniposide conditioned medium group and ginsenoside-Rg1 conditioned medium group was significantly increased

LDH leakage rate and expression of neuron NMDAR1 was significantly decreased in Tongluo Jiunao conditioned medium group and geniposide conditioned medium group（ P <0. 05 or P < 0. 01） and the expression of caspase-3 was significantly decreased in Tongluo Jiunao conditioned medium group and ginsenoside-Rg1 conditioned medium group（ P < 0. 05 or P < 0. 01）. Conclusion Tongluo Jiunao Injection and its monomer composition（ efficient compositions of gardenia and panax notoginseng） can decrease LDH leakage rate and expressions of NMDAR1 and caspase-3

enhance the viability of neurons and reverse the possible impact of microglia damage on neurons by regulating the secretion of microglia. The neurons may be protected. In addition

Tongluo Jiunao Injection has the therapeutic advantage of integrated regulation.

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