Objective To observe the influence of traditional Chinese medicine（ TCM） constitution differentiation and cultivation on growth and development of ovarian follicle in infertility patients with ovulatory dysfunction.Methods Thirty-two infertility patients diagnosed with ovulatory dysfunction were investigated with cross sectional questionnaire. Information on patient’s TCM constitution was collected

and constitution differentiation and classification were conducted. Then corresponding TCM constitution intervention was applied. Growth and development of follicle

as well as ovulation was recorded. Results Anovulation and small follicle ovulation were commonly seen in these 32 patients. After intervention of TCM constitution differentiation and cultivation for 3 menstrual cycles

17 cases（ 53. 13%） showed normal ovulation. Before treatment

average size of ovarian follicle of patients was（ 1. 47 ±0. 45） cm

but it was（ 1. 70 ± 0. 38） cm after TCM intervention

which showed statistical significance（ P < 0. 05）.Conclusion Intervention of TCM constitution differentiation and cultivation can improve growth and development of ovarian follicle in patients with ovulatory dysfunctional infertility and benefit ovulation.