Objective To study the gene expression differences and to explore potential target genes in infertility with different types of kidney deficiency syndromes with genomics methods. Methods Infertile female patients undergone in vitro fertilization-embryo transfer / intracytoplasmic sperm injection（ IVF-ET / ICSI-ET） with kidney-yin deficiency syndrome

with kidney-yang deficiency syndrome

or in normal conditions（ infertility caused by male factors） were selected respectively

with 33 cases in each group. On oocytes collecting day

follicular fluid in the follicles with the diameter of 1. 80 to 2. 00 cm was extracted in the 3 groups to get granulosa cells. High-throughput sequencing technology was applied to detect gene expressions

and to analyze differentially expressed genes

their functions and pathways. Results Compared with the normal group

the gene expression profiles in the kidney-yin deficiency group and kidney-yang deficiency group showed significant differences. There were 313 genes expressed differently in kidney-yin deficiency group from those in the normal group

with 219 up-regulating and 94 downregulating. And there were 319 genes expressed differently in kidney-yang deficiency group

with 218 up-regulating and 101 down-regulating. Gene ontology（ GO） of differentially expressed genes and the signal transduction pathway analysis showed that individually regulated genes of kidney-yin deficiency syndrome were mainly related to cell apoptosis and reproductive function

while that of kidney-yang deficiency syndrome were associated with pregnancy and embryo implantation. Conclusion p53

Bax

Bcl-2

IGFBP7

HSL and RPL29 are related to cell apoptosis

reproductive function

pregnancy and embryo implantation. These genes are likely to be the target genes for kidney deficiency syndrome differentiation in infertility.