Objective To study the possible mechanism of Chuanjiao Formula（ CJF） in the treatment of allergic conjunctivitis（ AC）. Methods Totally 20 SD rats were randomlly divided into the blank group

the low

medium

and high dose CJF groups

each with 5 rats. Rats in the low

medium

and high dose CJF groups were given CJF 20 ml / kg at the dose of 0. 334

0. 668

1. 336 g / ml by gavage

twice a day for 3 days. Rats in the blank group were given same amount of normal saline by gavage

twice a day for 3 days. Then drug-containing serum was made. The optimal concentration of serum on rat basophilic leukemia cells（ RBL-2H3） was screened and the optimal action time were screen as well. Experiments subdivided into model group

high

medium and low dose CJF group

tyrosine kinase inhibitor（ PP2） treatment group and control group. The low

medium

and high dose CJF groups were given drug-containing serum 40 μl each well using the screened serum concentration and time. PP2 treatment group were given 40 μl of PP2 with the concentration of 1 μM 30 min before adding the antigen. The control group were given 10% blank serum 40 μl 2 hours before adding the antigen. Enzyme-linked immunosorbent assay（ ELISA）was applied to detect β- hexosaminidase release rate. Results With the concentration of 10% CJF contained serum

cells showed better viability

so in this study

the concentration of 10% was chosen as the experimental concentration. Cell incubation with serum 120 min in medium

and high dose CJF groups showed the least degranulation（ P < 0. 01）

so 120 min was chosen as the incubation time. Compared with the normal group

β-hexosaminidase release rates of the model group and the control group increased significantly（ P < 0. 01）. The β-hexosaminidase release rates of each dose CJF group and PP2 treatment group declined（ P < 0. 01）. Conclusion The mechanism of CJF in treating allergic conjunctivitis may be related to the inhibition of degranulation in mast cells.