Objective To explore possible action mechanism of Tangluoning Extract in curing Diabetic peripheral neuropathy（ DNP）. Methods Diabetic rat model was induced by injecting a high-dose streptozotocinto into abdominal cavity. The successful model rats were randomly divided into model group

traditional Chinese medicine group

and western medicine group

with 23 rats in each group. Another 15 healthy rats were set as normal group.Traditional Chinese medicine group was perfused with crude drugs of Tangluoning Extract 20 g /（ kg·d）. Western medicine group was perfused with α-lipoic acid 20 mg /（ kg·d）. Normal and model groups were perfused with the same volume of distilled water as the criteria of body volume. The gavage lasted for 12 weeks. Levels of malondialdehyde（ MDA）

activity of superoxide dismutase（ SOD） in serum and sciatic nerve

and expression of protein of p38 MAPK

p-p38 MAPK

p46 JNK

p-p46 JNK

ERK1 /2

and p-ERK1 /2 in dorsal root ganglions（ DRG） of rates in each group were observed. Results Activity of SOD in serum and sciatic nerve decreased in model group

but levels of MDA in model group increased remarkably compared with that in normal group（ P < 0. 01）; Activity of SOD in serum and sciatic nerve increased

but contents of MDA decreased remarkably in both traditional Chinese medicine and western medicine groups compared with model group（ P < 0. 01）. Expression of total protein of p38 MAPK

p-p38 MAPK

p46 JNK

p-p46 JNK

ERK1 /2

and p-ERK1 /2 in DRG in each group were similar to each other.There was no significant difference between groups（ P > 0. 05）. Expression of active protein of p38 MAPK

p-p38 MAPK

p46JNK

p-p46 JNK

ERK1 /2

and p-ERK1 /2 in DRG in model group increased remarkably compared with that in normal group（ P > 0. 05）. But expression of active protein of p38 MAPK

p-p38 MAPK

p46 JNK

p-p46 JNK

ERK1 /2

and p-ERK1 /2 in DRG in both traditional Chinese medicine and western medicine groups decreased remarkably compared with model group（ P < 0. 05）. Conclusion Tangluoning Extract is effective in decreasing contents of MDA in serum and sciatic nerve and increasing activity of SOD in serum and sciatic nerve

by which oxidative stress response is inhibited

and MAPK signal transduction pathway is negatively regulated.