Objective To observe the effect of quercetin in different concentration on apoptosis of human hepatoma cell line Hep3 B and Huh7. Methods Human hepatoma cell line Hep3 B and Huh7 were planted in 96-well plate respectively

with 2 × 105 cells in each orifice. Six parallel holes were designed. The plate was put in 37℃ and 5%CO2incubator until the cells grew adhering to the wall. Quercetin in different concentration（ 0

10

50

and100 μM）was added in. After 48 h

cells were collected with trypsin. Hep3 B and Huh7 cells cycle were analyzed using flow cytometry after adding quercetin in different concentration. Results When the concentration of quercetin was 10

50

or 100 μM

the percentage of Hep3 B and Huh7 rose with the concentration increasing in Pre-G0 phase

and the top percentage reached when quercetin concentrastion was 100 μM（ P < 0. 01）. The percentage of Hep3 B and Huh7 decreased significantly after adding quercetin in different concentration in G1

S and G2 / M phase. Conclusion Quercetin could induce apoptosis of human hepatoma cell line Hep3 B and Huh7

and with the concentration of100 μM

the effect is the best.