Objective To observe the effect of Chaiqin Chengqi Tang on expression of interleukin-1β（ IL-1β）in pulmonary macrophage of dogs with acute necrotic pancreatitis（ ANP）. Methods Fifteen beagle dogs were randomly assigned into control group

model group and Chaiqin Chengqi Tang group

with five dogs in each group.50 mg / kg of sodium taurocholate was retrograde injected through bile duct to establish ANP model. Dogs in control and model groups were administered with 10 ml / kg of saline via gastric tubes

while those in Chaiqin Chengqi Tang group were administered with Chaiqin Chengqi Tang in the same dosage and delivery route. Every group was administered one time per two hours

for three times. In the sixth hour

canine serum was collected for detecting interleukin-1β（ IL-1β）and pulmonary tissue was obtained to isolate macrophages for detecting IL-1β mRNA and protein expression. Results IL-1β mRNA expression in pulmonary macrophages was significantly decreased in model and Chaiqin Chengqi Tang groups compared with that in control group（ P < 0. 05）. IL-1β protein and serum IL-1βexpressions in pulmonary macrophages were significantly higher in model group than those in control and Chaiqin Chengqi Tang groups（ P <0. 05）. Conclusion Chaiqin Chengqi Tang can decrease the IL-1β protein expression of canine pulmonary macrophages

leading to the reduction of serum IL-1β.