Objective To explore the possible functional mechanism of ganoderan in improving anti-tumor immune response. Methods Melanoma cells of mice B16F10 cultured in vitro were treated using ganoderan

and were divided into low（ 200 μg / ml）

middle（ 400 μg / ml）

and high（ 600 μg / ml） dosage groups according to the ganoderan concentration

while RPMI-640 nutrient solution was used as the control group. Mice spleen lymphocytes 1 ×106/ hole were added into ganoderan treated B16F10 cells

and after being cultured for 18 h or five days

respectively

the expression of activated molecule of CD69 and FASL on the surface of spleen lymphocytes were examined respectively. Mice spleen lymphocytes 5 × 105/ hole were added into Gl-PS treated B16F10 cells

and after being cultured for 5 days

the content of IFN-γ was detected using ELISA method. Results CD69 levels in low

middle and high dosage groups were remarkably higher than that in control group（ P < 0. 05）. FASL levels in middle and high groups were remarkably higher than that in control group（ P < 0. 05）; and FASL levels in middle and high groups were remarkably higher than that in low group（ P < 0. 05）. There were no statistically significant differences in CD 69 levels between each dosage group（ P > 0. 05）. IFN-γ level in low

middle and high dosage groups were remarkably higher than that in control group（ P < 0. 05）. IFN-γ level in middle dosage group was remarkably higher than that in low and high dosage groups（ P < 0. 05）. Conclusion Ganoderan could improve anti-tumor immune immunocompetence of lymphocytes

which may had a relationship with activating the expression of CD69 and FASL of mice spleen lymphocytes and improving secretion of IFN-γ.