益气凉血生肌方对血管内皮细胞损伤后细胞形态学的影响

苏菲; 孙娅楠; 崔晓云; 林谦

doi:10.13288/j.11-2166/r.2015.19.019

您当前的位置：

首页 >

文章列表页 >

益气凉血生肌方对血管内皮细胞损伤后细胞形态学的影响

实验研究 | 更新时间：2021-11-17

- 益气凉血生肌方对血管内皮细胞损伤后细胞形态学的影响
- The Effects of Yiqi Liangxue Shengji Formula（益气凉血生肌方） on Cell Morphology of Injured Vascular Endothelial Cells
- 中医杂志 2015年56卷第19期页码：1691-1694
- 作者机构：
  
  1. 北京中医药大学
  2. 中国中医科学院医学实验中心
  3. 北京中医药大学东方医院
- 作者简介：
- 基金信息：
  
  北京中医药大学薪火传承研究项目（教-01101）
- DOI：10.13288/j.11-2166/r.2015.19.019
  中图分类号： R285.5
- 纸质出版：2015
- 稿件说明：
移动端阅览
[1]苏菲,孙娅楠,崔晓云,林谦.益气凉血生肌方对血管内皮细胞损伤后细胞形态学的影响[J].中医杂志,2015,56(19):1691-1694.

SU Fei, SUN Yanan, CUI Xiaoyun, et al. The Effects of Yiqi Liangxue Shengji Formula（益气凉血生肌方） on Cell Morphology of Injured Vascular Endothelial Cells[J]. Journal of Traditional Chinese Medicine2015, 56(19): 1691-1694.
[1]苏菲,孙娅楠,崔晓云,林谦.益气凉血生肌方对血管内皮细胞损伤后细胞形态学的影响[J].中医杂志,2015,56(19):1691-1694. DOI： 10.13288/j.11-2166/r.2015.19.019.

SU Fei, SUN Yanan, CUI Xiaoyun, et al. The Effects of Yiqi Liangxue Shengji Formula（益气凉血生肌方） on Cell Morphology of Injured Vascular Endothelial Cells[J]. Journal of Traditional Chinese Medicine2015, 56(19): 1691-1694. DOI： 10.13288/j.11-2166/r.2015.19.019.

摘要

目的从血管内皮细胞形态学方面探讨益气凉血生肌方降低冠心病介入术后远期心血管事件的作用机制。方法 20只大白兔随机分为正常组、生理盐水组、中药组、西药组和中西医结合组

每组4只。中药组灌胃益气凉血生肌方2.3 g/（kg·d）

西药组灌胃氯沙坦钾片0.115 g/（kg·d）

中西医结合组间隔30 min分别灌胃等剂量的益气凉血生肌方和氯沙坦钾片

生理盐水组灌胃等量0.9%Na Cl溶液

正常组不做任何处理。连续灌胃5天后耳缘静脉采血

无菌分离血清。采用体外实验的方法

对人脐静脉内皮细胞（HUVECs）采用血管紧张素Ⅱ（AngⅡ）建立细胞损伤模型

分为对照组、模型组、中药组、西药组、中西医结合组

依次加入相应的0.15 ml 15%药物血清。培养箱培养4

h后采用倒置显微镜观察细胞整体形态变化、透射电镜观察细胞超微结构变化。结果光镜下模型组细胞较对照组形态紊乱、生长缓慢

进行药物干预后

细胞生长加快

形态恢复

中西医结合组更明显。透射电镜下与模型组比较

中药组、西药组和中西医结合组损伤内皮细胞的细胞核边界清楚、内质网增多增密、线粒体数目增加

中药组和中西医结合组的内质网折叠更明显

线粒体饱满、数目较西药组增多。结论益气凉血生肌方在形态学上对损伤性血管内皮细胞有促进修复的作用

能保护血管内皮的完整性

从而减轻冠状动脉介入治疗对内皮细胞的损伤。

Abstract

Objective To study the mechanism of Yiqi Liangxue Shengji Formula in decreasing long term cardiovascular events after percutaneous coronary intervention from the view of vascular endothelial cell morphplogy.Methods Randomly divided 20 rabbits into normal group

normal saline group

Chinese medicine group

western medicine group and integrat medicine group

with 4 rabbits in each group. Perfused Chinese medicine group with Yiqi Liangxue Shengji Formula 2. 3 g /（ kg·d）

western medicine group with losartan pills 0. 115 g /（ kg·d）

Chinese and western medicine group respectively with equal dosage of Yiqi Liangxue Shengji Formula and losartan pills at 30-minutes intervals. Perfused normal saline group with equal dosage of Na Cl solution. No treatment was given to normal group. Collected blood sample from ear marginal veins after perfusing for 5 days continuously. Aseptically separated serum. Adopting angiotensin Ⅱ（ Ang Ⅱ） to establish cell injury model on human umbilical vein endothelial cells（ HUVECs）

and divided them into control group

model group

Chinese medicine group

Chinese and western medicine group. Added successively corresponding 0. 15 ml 15% medicated serum. Adopting inverted microscope to observe integral form changes of cells after culturing in incubator for 4 to 6 hours

and adopting transmission electron microscope to observe ultrastructure changes in cells. Results Under optical microscope

model group cells grew more slowly with more disordered form. After medicine intervention cells grow faster with form recovering

that of Chinese medicine and western medicine group was more remarkable. By transmission electron microscope

Chinese medicine group

western medicine group

Chinese and western medicine group injured vascular endothelial cells nucleus boundary was explicit

endoplasmic reticulum increased and densified

the number of mitochondrion increased

compared with model group. Chinese medicine group

Chinese and western medicine group endoplasmic reticulum fold more obvious

mitochondrion was full

with its number was more than western medicine group.Conclusion Yiqi Liangxue Shengji Formula has the function of promoting repairing the injured vascular endothelial cells in morphology. It can protect the integrity of endothelial cell

which can decrease the injury of coronary artery intervention therapy to endothelial cells.

关键词

Keywords

references

查看原文

浏览量

184

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

温心方含药血清对人脐静脉内皮细胞外泌体及人主动脉血管平滑肌细胞表型转化的影响

益气凉血生肌方预防冠心病PCI术后气虚血瘀、痰热互结证患者主要不良心血管事件的随机对照试验

从“内风暗旋”辨治小细胞肺癌

真实世界研究视角下功能性消化不良-肠易激综合征共病随机对照试验的现状分析及对策建议

基于病络理论探讨类风湿关节炎合并冠状动脉粥样硬化性心脏病的共病机制及防治策略