Objective To investigate and analyze the influential factors of TCM constitution. Methods The researchers used a cross-sectional survey method to make on-site questionnaire survey on 1627 freshmen of 2010 class in Henan University of Traditional Chinese Medicine. Investigation included basic situation and scale of TCM constitution. Survey data were collected to establish a database. The researchers used Classification and Identification of Traditional Chinese Medicine Constitution to identify constitution category of subjects and used Logistic multiple regression to analyze influence factor of constitution. Results Regular sleep was negatively correlated

while gender

light taste

past medical history and allergic history were positively correlated with constitution of Qi deficiency.Cold food preference and regular sleep were negatively correlated

however

gender and allergic history were positively correlated with constitution of Yang deficiency. Age was negatively correlated

however gender

pungency taste preference

past medical history and allergic history were positively correlated with constitution of Yin deficiency.Regular sleep was negatively correlated

however

sweet taste preference

sour taste preference

tobacco

past medical history and allergic history were positively correlated with constitution of phlegm-damp deficiency. Age and regular sleep were negatively correlated

however sweet taste preference

pungency taste preference

tobacco were positively correlated with constitution of dampness-heat. Territory distribution area was negatively correlated

however gender

allergic history and family history were positively correlated with constitution of blood stasis. Regular sleep was negatively correlated

however

gender and allergic history were positively correlated with constitution of Qi depression.Salty taste preference and regular sleep were negatively correlated

however territory distribution area

greasy food preference and allergic history were positively correlated with constitution of specific endowment（ P < 0. 05）.Conclusion Gender

place of residence

diet and daily life

past medical history and allergic history are all the influence factors of TCM constitution of freshmen. Among theses

pungency taste preferenc is the risk factor leading to constitution of Yin deficiency

while sweet taste preference and tobacco are the risk factors leading to constitution of phlegm-damp and dampness-heat.