Objective To observe the effect and possible mechanism of Guizhi Tang（ 桂枝汤） on cardiac autonomic nerve remodeling of rats with diabetes. Methods Randomly divided 24 rats into normal control group

model control group

Methycobal group

and Guizhi Tang group

with 8 rats each. All groups except the normal control group adopted one-time intraperitoneal injection Streptozotocin to build diabetes model. The Methycobal group was perfused with Methycobal suspension 0. 15 mg /（ kg·d）. The Guizhi Tang group was given Guizhi Tang 4 g /（ kg·d）for gavage. The normal control group and the model control group were given equal dose normal saline for gavage respectively. All groups were perfused for 7 weeks. Adopted physiological parameters radiotelemetry system to examine electrocardiogram and calculated heart rate variability（ HRV） of rats

including standard diviation of NN intervals（ SDNN）

root mean square of sussessive differences（ RMSSD）

standard diviation average of NN intervals（ SDANN） every 5 min of time-domain analysis

and low frequency（ LF）

high frequency（ EF） and total power（ TP） and LF/EF of frequency domain analysis. Examined content of tyrosine hydroxylase（ TH）

Choline acetyl amino transferase（ Ch AT）

growth-associated protein 43（ GAP-43）

neurotrophic factors（ NGF） and ciliary neurotrophic factor（ CNTF） in homogegate of interventricular septum and left ventricle. Results SDNN

RMSSD

LF

HF and TP of rats in the Guizhi Tang group increased while SDANN

LF / HF decreased compared with that of the model control group. Ch AT increased and TH / Ch AT decreased of interventricular septum and left ventricle of rats.GAP-43 and CNTF of interventricular septum and left ventricle increased while NGF of interventricular septum decreased（ P < 0. 05 or P < 0. 01）. SDANN

LF / HF of rats in the Guizhi Tang group decreased

RMSSD

LF

HF and TP increased compared with that of the Methycobal group. Ch AT

GAP-43

CNTF of interventricular septum and left ventricle increased

while TH / Ch AT decreased（ P < 0. 05 or P < 0. 01） Conclusion Guizhi Tang could prevent cardiac autonomic nerve injury of diabetes

relieve autonomic nerve remodeling. Its mechanism may be related to regulating sympathetic nerve and vagus nerve banlance.