Objective To study the possible functional mechanism of Weikangning in treating functional dyspepsia from aspect of energy metabolism. Methods After siRNA of 3 target voltage-independent negative ion channels proteins（ VDAC1） were transferred to human gastric epithelial cells GES-1

the possible silence efficiency of VDAC1 was examined. DDK-Myc plasmid and VDAC1-DDK-Myc plasmid were transferred

and the over-expression efficiency of VDAC1 was examined. GES-1 cells（ 1 × 10

4

cells / well） were put into a 96 well plate

and divided into the blank control group

the positive control group

and the Weikangning low

middle and high dose groups

using 5 parallel holes in each. No medicine was added into the blank control group. After cells silencing or over-expressing

2. 5 μmol of Domperidone Tablets were added into the positive control group

while 0. 025 μg / μl

0. 100 μg / μl

or 0. 300 μg /μl of Weikangning were added into the Weikangning low

middle and high dose groups

respectively. The ATP levels in cells of each group were examined using bioluminescence method before and after cells silencing and expressing.Results Transferring using siRNA1 could decrease the level of VDAC1 gene and protein. Transferring DDK-Myc plasmid and VDAC1-DDK-Myc plasmid could both increase the level of VDAC1 protein. There were statistically significant differences in those decreases and increases between the Weikangning groups and the control groups（ P

<

0. 01）. The ATP levels in cells of each group decreased after VDAC1 silencing compared with that before silencing（ P

<

0. 05 or P

<

0. 01）

increased remarkably after VDAC1 over-expressing compared with that before over-expressing（ P

<

0. 05）. The ATP levels in cells of Weikangning middle dose group remarkably increased after VDAC1-DDKMyc transferred compared with that of the blank control group（ P

<

0. 05）. Conclusion Weikangning may treat functional dyspepsia through promoting the energy metabolism of gastric epithelial cells.