左归丸、右归丸及其拆方对去卵巢骨质疏松症模型大鼠肾脏碱性磷酸酶、骨钙素表达的影响

孟玥; 任艳玲; 孙月娇; 李书娟

doi:10.13288/j.11-2166/r.2016.05.016

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左归丸、右归丸及其拆方对去卵巢骨质疏松症模型大鼠肾脏碱性磷酸酶、骨钙素表达的影响

实验研究 | 更新时间：2021-11-17

- 左归丸、右归丸及其拆方对去卵巢骨质疏松症模型大鼠肾脏碱性磷酸酶、骨钙素表达的影响
- Impact of Zuogui Pill（左归丸）,Yougui Pill（右归丸） and Their Decomposed Recipes on the Expression of Renal Alkaline Phosphatase and Osteocalcin in Ovariectomized Osteoporosis Model Rats
- 中医杂志 2016年57卷第5期页码：423-427
- 作者机构：
  
  辽宁中医药大学
- 作者简介：
- 基金信息：
  
  国家自然科学基金（81373527）
- DOI：10.13288/j.11-2166/r.2016.05.016
  中图分类号： R285.5
- 纸质出版：2016
- 稿件说明：
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[1]孟玥,任艳玲,孙月娇,李书娟.左归丸、右归丸及其拆方对去卵巢骨质疏松症模型大鼠肾脏碱性磷酸酶、骨钙素表达的影响[J].中医杂志,2016,57(05):423-427.

MENG Yue, REN Yanling, SUN Yuejiao, et al. Impact of Zuogui Pill（左归丸）,Yougui Pill（右归丸） and Their Decomposed Recipes on the Expression of Renal Alkaline Phosphatase and Osteocalcin in Ovariectomized Osteoporosis Model Rats[J]. Journal of Traditional Chinese Medicine2016, 57(5): 423-427.
[1]孟玥,任艳玲,孙月娇,李书娟.左归丸、右归丸及其拆方对去卵巢骨质疏松症模型大鼠肾脏碱性磷酸酶、骨钙素表达的影响[J].中医杂志,2016,57(05):423-427. DOI： 10.13288/j.11-2166/r.2016.05.016.

MENG Yue, REN Yanling, SUN Yuejiao, et al. Impact of Zuogui Pill（左归丸）,Yougui Pill（右归丸） and Their Decomposed Recipes on the Expression of Renal Alkaline Phosphatase and Osteocalcin in Ovariectomized Osteoporosis Model Rats[J]. Journal of Traditional Chinese Medicine2016, 57(5): 423-427. DOI： 10.13288/j.11-2166/r.2016.05.016.

摘要

目的探讨左归丸、右归丸治疗绝经后骨质疏松症的可能作用机制。方法将90只SD雌性大鼠随机分为空白组、假手术组、模型组、补佳乐组、左归丸组、右归丸组、共同药组、滋阴组、补阳组各10只。除假手术组、空白组外

其余各组均进行双侧卵巢切除制备绝经后骨质疏松症大鼠模型。造模1周后

补佳乐组、左归丸组、右归丸组、共同药组、滋阴组、补阳组每只大鼠每天灌胃相应药物

剂量分别为0.36 mg/（kg·d）、18.9 g/（kg·d）、20.52 g/（kg·d）、15.12 g/（kg·d）、12.96 g/（kg·d）、8.1 g/（kg·d）

空白组、假手术组、模型组灌胃等量蒸馏水

各组均灌胃12周。检测各组大鼠肾组织中碱性磷酶（ALP）、骨钙素（BGP）mRNA和蛋白表达

取大鼠全股骨进行骨密度检测。结果与空白组及假手术组比较

模型组大鼠肾组织ALP、BGP mRNA及蛋白表达和全股骨密度均有所下降（P<0.01）;与模型组比较

除共同药组外其余各用药组大鼠肾组织ALP、BGP mRNA及蛋白和全股骨密度表达均有不同程度提高（P<0.05或P<0.01）

其中以补佳乐组、左归丸组效果最好。结论左归丸、右归丸及其拆方可能通过提高去卵巢骨质疏松症模型大鼠肾组织ALP、BGP的表达从而有效治疗绝经后骨质疏松症

并且以左归丸疗效更佳。

Abstract

Objective To explore the possible mechanism of Zuogui Pill（左归丸） and Yougui Pill（右归丸） in the treatment of postmenopausal osteoporosis（ PMOP）. Methods Ninety Sprague Dawley female rats were randomized into the blank control group

the sham operation group

the model group

the progynova group

the Zuogui Pill group

the Yougui Pill group

the common drug group

the nourishing Yin group

and the invigorating Yang group（ n = 10 in each group）. Rats in other groups were established models of PMOP by bilateral ovariectomy except rats in the sham operation group and the blank control group. After models were established for 1 week

the progynova group

the Zuogui Pill group

the Yougui Pill group

the common drug group

the nourishing Yin group and the invigorating Yang group were given corresponding drugs by gavage

with the dose of 0. 36 mg /（ kg·d）

18. 9 g /（ kg·d）

20. 52 g /（ kg·d）

15. 12 g /（ kg·d）

12. 96 g /（ kg·d） and 8. 1 g /（ kg·d）

respectively for 12 weeks. The blank control group

the sham operation group and the model group were given equivalent distilled water by gavage for the same time. Alkaline phosphatase（ ALP）

bone gla-protein（ BGP） mRNA and protein expression in renal tissue of rats in all groups were detected. Bone mineral density（ BMD） of whole femur was detected. Results Compared with the blank control group

renal tissue ALP

BGP mRNA

protein expression and BMD of whole femur decreased in the model group（ P < 0. 01）. Compare with the model group

renal tissue ALP

BGP mRNA

protein expression and BMD of whole femur in all medication groups increased by different level（ P < 0. 05 or P < 0. 01）. The most effective groups were the progynova group and the Zuogui Pill group. Conclusion Zuogui Pill

Yougui Pill and their decomposed recipes might treat PMOP effectively by improving the expression of renal tissue ALP and BGP in ovariectomized osteoporosis model rats. Zuogui Pill is more effective.

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