LI Changming, XIE Shangju, QUAN Renfu, et al. Effects of Elongated Needle with Paired Needling “Shuidao”(ST28) and “Zhibian”(BL54) on Neurological Function of Spinal Cord Injury Rats[J]. Journal of Traditional Chinese Medicine2016, 57(6): 516-519.
LI Changming, XIE Shangju, QUAN Renfu, et al. Effects of Elongated Needle with Paired Needling “Shuidao”(ST28) and “Zhibian”(BL54) on Neurological Function of Spinal Cord Injury Rats[J]. Journal of Traditional Chinese Medicine2016, 57(6): 516-519.DOI: 10.13288/j.11-2166/r.2016.06.018.
Objective To observe effects and possible mechanism of elongated needle with paired needling"Shuidao"( ST28) and"Zhibian"( BL54) on neurological function of spinal cord injury rats. Methods Thirty SD rats were randomly divided into control group
model group and treatment group
10 rats in each group. The model group and the treatment group were used improved Allen ’s method to make models of spinal cord injury. In the treatment group
when rats revived after operation
the researchers immediately used elongated needle to needle"Shuidao"( ST28) and "Zhibian"( BL54) with method of paired needling to acupuncture the rats
about 2 cm depth and twirl for 1 min and made two ipsilateral acupoints to form a loop connected to electric acupuncture apparatus
electric acupuncture for 30 min
once per day. After 5 days the researchers used BBB scoring method to observe motor function of rats’ hind limbs and took tissue of spinal cord to make histological observation
count apoptotic index and detect expression of the Bcl-2 and Bax. Results Compared with the control group
in the model group
BBB motor function score significantly decreased but nerve cell apoptotic index and percentage of positive cells about Bax and Bcl-2 of spinal cord tissue increased( P < 0. 05 or P < 0. 01). Compared with the model group
in the treatment group
BBB motor function score significantly increased but nerve cell apoptotic index decreased( P < 0. 05 or P < 0. 01); And percentage of positive cells about Bax of spinal cord tissue significantly decreased but percentage of positive cells about Bcl-2 significantly increased( P < 0. 01). Conclusion Elongated needle with paired needle"Shuidao"( ST28) and"Zhibian"( BL54) may promote neural functional to recover by regulating the expression of apoptotic gene that Bax and Bcl-2 to reduce apoptosis of nerve cells.