Objective To explore the diagnostic marker of primary nephrotic syndrome（ PNS） and the therapeutic target of Shenkangling（ 肾康灵） decoction. Methods Fifty SD rats were randomized into normal group

model group

prednisone group

the herb-western medicine group and the Shenkangling group

10 in each group. Except for the normal group rats in other groups were injected adriamycin 5. 5 mg / kg by tail vein on the first day of the test to establish adriamycin nephropathy rats models. One week after modeling

the normal group and the model group were given 3 ml distilled water in the morning and in the afternoon respectively by gavage. The prednisone group was given prednisone suspension 5. 5 mg / kg in the morning by gavage

3ml distilled water in the afternoon. The Shenkangling group was given Shenkangling decoction 1. 5 g /100 g in the morning by gavage

3ml distilled water in the afternoon. The herb-western medicine group was given prednisone suspension 5. 5 mg / kg in the morning by gavage

Shenkangling decoction 1. 5 g /100 g in the afternoon. After 22 days

urine was collected and differential proteins in all groups were examined by isobaric tags for relative and absolute quantitation（ i TRAQ） quantitative analysis technology combined with mass spectrometric method. Results There were three significantly differential proteins in the model group and the normal group

including highly expressed protein afamin

isoform CRA

a

as well as lowly expressed proteins gro and Cystatin related protein 2. Compared the herb-western medicine group with the model group

there were six significantly differential proteins

including highly expressed proteins apolipoprotein A-Ⅱ

Apolipoprotein A-Ⅳ

Vitronectin

osteopontin and Coagulation factor Ⅻ

as well as lowly expressed protein afamin

isoform CRA

a. Compared the Shenkangling group with the model group

there were six significantly differential proteins

including highly expressed proteins apolipoprotein A-Ⅱ

Complement factor B

Vitronectin

osteopontin and Coagulation factor Ⅻ

as well as lowly expressed protein afamin

isoform CRA

a. Conclusion Gro

Cystatin related protein 2

afamin and isoform CRA

a might be used as the diagnostic markers of PNS. Afamin

isoform CRA

a

apolipoprotein A-Ⅱ

Complement factor B

Vitronectin

osteopontin and Coagulation factor Ⅻ might be used as the therapeutic target of Shenkangling.