Objective To investigate the association between the gene polymorphism of mitogen activated protein kinase 4（ MAP2K4） and mitogen activity protein kinase 1（ MAPK1） and the susceptibility of ischemic stroke with wind phlegm and blood stasis syndrome

Qi deficiency and blood stasis syndrome and the blood lipid level. Methods A case control study method was applied

and there were 311 cases of ischemic stroke with phlegm and blood stasis syndrome

284 cases with Qi deficiency and blood stasis syndrome

and 605 cases of normal control subjects included.Gene typing was performed by using Sequenom technology

the expression level of MAP2K4 and mRNA of MAPK1 in peripheral blood and the blood lipid level were detected by real-time fluorescence quantitative PCR（ RT-PCR）.Results The MAPK1 gene rs9340 polymorphism was significantly associated with the susceptibility to wind phlegm and blood stasis syndrome in male patients with ischemic stroke（ P = 0. 047）

however there was no association between MAPK1 gene rs6928 polymorphism and MAP2K4 gene rs3826392 polymorphism and the susceptibility of ischemic stroke with wind phlegm stasis syndrome

and Qi deficiency and blood stasis syndrome（ P > 0. 05）. The expression level of mRNA MAPK1 was significantly lower in patients with wind phlegm and blood stasis syndrome carrying rs6928 CC genotype than other genotype carriers（ P = 0. 006）. In ischemic stroke patients with Qi deficiency and blood stasis syndrome

MAPK1 gene rs6928 polymorphism was significantly associated with levels of triglyceride（ TG） and high density lipoprotein（ HDL）（ P < 0. 05）

and there was significant association between rs9340 and TG levels（ P < 0. 05）

but the association between MAP2K4 gene rs3826392 polymorphism and the blood lipid levels in ischemic stroke patients with phlegm and blood stasis syndrome

Qi deficiency and blood stasis syndrome were not found（ P > 0. 05）. Conclusion MAPK1 gene rs9340 polymorphism may affect the occurrence of ischemic stroke with wind phlegm and blood stasis syndrome in Chinese male. MAPK1 gene polymorphism affects the blood lipid metabolism in ischemic stroke with Qi deficiency and blood stasis.