Objective To investigate the effects of Naoluo Xintong Compound Formula（脑络欣通复方） on angiogenesis and local relative cerebral blood flow（rCBF） in rats with middle cerebral artery occlusion / reperfusion（ MCAO /R） and its possible mechanism. Methods One hundred and sixty Wistar rats in total were used in the study. Seventy Wistar rats were randomly divided into a normal group

a low dose normal group

a middle dose normal group

a high dose normal group

a low dose induced group

a middle dose induced group

and a high dose induced group

with 10 rats in each group. Rats in the different dose normal groups and induced groups were given400 mg / kg

800 mg / kg

or 1000 mg / kg of Naoluo Xintong Compound Formula solution 1 ml /（ 100 g·d） for gavage

respectively. The rats in each induced group were injected with 50% CCl4 olive oil solution after 7 days of continuous gavage. Twenty-four h after inducing completed

liver function of rats

including serum levels of alanine aminotransferase（ ALT）

aspartate amino transfer enzyme（ AST）

lactate dehydrogenase（ LDH）

and albumin（ ALB） were tested to select safe dose. Another 90 Wistar rats were randomly divided into a sham operation group

a model group and a Naoluo Xintong Compound Formula group

with 30 rats in each group. MCAO / R model was made with thread embolism method in model group and Naoluo Xintong group. According to the results from the above safe dose experiment

safety dose of Naoluo Xintong liquid were given for gavage. On the 3rd

7th

and 14 th day of gavage

levels of homogenates thrombopoietin（ TPO） and endothelial（ ET）

as well as expression of vascular endothelial growth factor（ VEGF） in brain were tested

and dynamic observation r CBF was observed in each group. Results Compared with that in the normal group

low dose normal group

and middle dose normal group

the AST and ALT both increased

Alb and LDH decreased in high dose normal group and each of induced groups（ P < 0. 05 or P < 0. 01）; Compared with that in the middle dose induced group

levels of ALT and AST increased

while Alb level decreased in the high and low dose induced groups（ P < 0. 01）. Therefore

the middle dose of 800 mg / kg was the safe dose. Compared with that in the sham operation group

levels of TPO and ET significantly increased

expression of VEGF increased

and r CBF decreased in model group（ P < 0. 01）; Compared with that in the model group

levels of TPO and ET significantly decreased

expression of VEGF increased

and r CBF was significantly improved（ P < 0. 05 or P < 0. 01） in Naoluo Xintong group. Comparison between different time points in Naoluo Xintong group showed that

levels of TPO and ET significantly decreased on the 7th and 14 th day compared with those on the 3rd day; expression of VEGF protein significantly increased on the 7th and 14 th day compared with that on the 3rd day; and r CBF significantly increased on the 14 th day compared with that on the 3rd day（ P < 0. 05 or P < 0. 01）. Conclusion Naoluo Xintong Compound Formula

by regulating coagulation fibrinolysis regulation factor and VEGF

as well as promoting angiogenesis in the model rats

could improve blood flow state

improve the regional cerebral blood flow

and save the ischemic penumbra to treat ischemic cerebrovascular disease.