Objective To investigate the possible mechanism of the collagen loading with Shengji Yuhong Onit-ment in promoting chronic ischemic wound healing. Methods Randomly divided 72 rats into the blank group

the control group and the experimental group

with 24 rats in each group. All rats were established into hind limb ischemic wound model.

with direct skin suture in the blank group

the oblong collagen（ size: 10 mm 10 mm × 2 mm）was loaded with the Shengji Yuhong extract（ 0. 5 g / ml

50 μl / each） in the experimental group and placed it at the ischemic muscle tissue in the blood vessel defect

with the same size collagen loaded with 50 μl normal saline in the ischemic muscle tissue in the blood vessel defect in the control group. Then the skin was sutured. Twenty-eight days were observed in each group. Observed the general situation of rats in each group

detected the microvessel count in the rat’ s ischemic wound hind limb and hemoglobin content in implanted samples in the control group and the experimental group on the 3 th

7 th

14 th day respectively. Results The rats in the blank group were hind limb claudication

partly pale and can see acrocyanosis

while rats in the experimental group had no obvious ischemic manifestations. Compared with the blank group

the microvessel count in the control group was higher than the blank group on the 14 th and 28 th day

and the microvessel count in the experimental group was significantly higher than that of the blank group on the 7 th

14 th and 28 th day（ P < 0. 05）. Compared with the control group

the microvessel count was significantly increased in the experimental group on the 7 th and 14 th day（ P < 0. 05）. The hemoglobin level of the implanted samples in the experimental group was significantly higher than that of the control group at the same time on the 3 rd

7 th

14 thand 28 th day（ P < 0. 05）. Conclusion The collagen loading with Shengji Yuhong ointment can promote angiogenesis in ischemic wound tissue and improve blood flow perfusion

which may the mechanism of promoting chronic ischemic wound healing.