Objective To summarize the rules of using the prescription and the herbs in Treatise on Blood Troubles. Methods Prescriptions recorded in Treatise on Blood Troubles were collected and coordinated. The names of prescriptions and herbs were typed to Auxiliary System of Chinese Medicine Inheritance V2. 5. The using frequency of herbs was calculated. Herb combinations

herb association and herb core combinations were analyzed by association rules

improved mutual information method and complex system entropy clustering. New prescriptions were analyzed by unsupervised entropy hierarchical clustering. Results In total

197 prescriptions were included

423 herbs used

with 2498 times of using frequency. Top 3 herbs with high using frequency were Gancao [Radix Glycyrrhizae]（103）

Danggui [Radix Angelicae Sinensis]（79） and Baishao [Radix Paeoniae Alba]（63）. Top 3 herb combinations with high using frequency were Gancao-Danggui（ 50）

Gancao-Baishao（ 42） and Baishao-Danggui（ 40）. Top3 correlation coefficients of herbs were Puhuang [Pollen Typhae]-Maidong [Radix Ophiopogonis ]（ 0. 02729976）

Wuweizi [Fructus Schisandrae Chinensis]-Wubeizi [Galla Chinensis ]（ 0. 02684237） and Wuweizi-Niuxi [Radix Achyranthis Bidentatae]（ 0. 02667364）. Based on complex system entropy clustering

28 groups of herb core combinations with 3- 4 herbs were developed. Fourteen new prescriptions were got by unsupervised entropy hierarchical clustering algorithm. Conclusion Herbs in Treatise on Blood Troubles were mainly lowering fire

regulating Qiflowing

harmonizing blood

reinforcing and dredging Zang-fu viscera. Cool and moisturizing natured herbs were used more

while heat and dry natured herbs were used less.