Objective To explore the possible mechanism of Sanhuang Yinchi Decoction（ 三黄茵赤汤） in treating acute liver injury. Methods Human embryonic liver cell line LO2 were divided into normal group

model group

control group and high

medium

and low dose Sanhuang Yinchi Decoction groups. D-galactosamine（ DGal N） was used to induce LO2 cells necrosis. The high

medium

and low dose Sanhuang Yinchi Decoction groups were given Sanhuang Yinchi Decoction 3

2

1 mg/ml respectively. The control group was given receptor interacting protein 1 inhibitor（ Nec-1） 60 mmol/L. The survival rate of cells in each group was determined by MTT method.Apoptosis and necrosis were analyzed by Hoechst and propidium iodide（ PI） staining. The apoptosis rate and the level of intracellular reactive oxygen species（ ROS） were detected by flow cytometry. The expressions of Casepase-3

receptor interacting protein1（ RIP1） and receptor interacting protein3（ RIP3） were detected by Western blot method.Results Compared with the model group

the survival rate of cells and ROS clearance in the medium and low dose Sanhuang Yinchi Decoction groups increased. The fluorescence intensity of Hoechst and PI staining in the mediumdose Sanhuang Yinchi Decoction group decreased. The ROS clearance in the high-dose Sanhuang Yinchi Decoction group increased（ P < 0. 05）. The effect of decreasing the late cell apoptosis rate and increasing the survival rate in the medium-dose Sanhuang Yinchi Decoction group was the best. The expressions of RIP1 and RIP3 in all dose Sanhuang Yinchi Decoction groups decreased（ P < 0. 05）. Compared with all dose Sanhuang Yinchi Decoction groups

the effect in the control group was more obvious（ P < 0. 01）. Conclusion Sanhuang Yinchi Decoction might have protection on LO2 cells necrosis induced by D-Gal N by inhibiting the expressions of RIP1 and RIP3.