Objective To compare the protective effects of Ganghuo Kanggan Decoction（ 岗藿抗感汤） containing natural bezoar

in vitro cultured bezoar

or artificial bezoar on mice infected by H1N1 influenza virus. Methods Ninety-six mice were randomized into a normal group

a model group

a ribavirin group

an artificial bezoar group

a natural bezoar group and a bezoar cultured in vitro group

16 mice in each group. Mice in other groups except the normal group were infected with H1N1 influenza virus liquid by nose drops. After mice were infected for 4 h

the ribavirin group was given 0. 2 ml/10 g of ribavirin tablet solution（ concentration 3. 75 mg/ml）. All bezoar groups were given 0. 2 ml/10 g of Ganghuo Kanggan Decoction containing bezoar from different sources（ concentration2. 31 g/ml）. The normal group and the model group were given equivoluminal normal saline

for 15 days. The average survival time

mortality protection rate

life extension rate

lung index

the ratio of lung wet-dry weight and relative expression of lung tissue virus were compared in all groups. Results The average survival time shortened

while the lung index

the ratio of lung wet-dry weight

and relative expression of lung tissue virus increased in the model group compared with those in the normal group（ P < 0. 01）. Compared with those in the model group

mice survival time increased

but lung index

the ratio of lung wet-dry weight and relative expression of lung tissue virus decreased in the ribavirin group

the artificial bezoar group

the bezoar cultured in vitro group

and the natural bezoar group（ P < 0. 05 or P < 0. 01）. There was no significant difference in the indexes among all bezoar groups（ P > 0. 05）.Conclusion Ganghuo Kanggan Decoction containing natural bezoar

bezoar cultured in vitro or artificial bezoar might increase survival time of mice infected by H1N1 influenza virus and reduce the lung tissue viral load. The difference of bezoar sources does not seem to influence the protection of Ganghuo Kanggan Decoction on mice infected by influenza virus.