左归丸联合温和灸对骨质疏松症模型大鼠骨密度、骨保护素mRNA及核因子κB受体活化因子配体mRNA的影响

钱康; 范永升

doi:10.13288/j.11-2166/r.2017.15.015

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左归丸联合温和灸对骨质疏松症模型大鼠骨密度、骨保护素mRNA及核因子κB受体活化因子配体mRNA的影响

实验研究 | 更新时间：2021-11-10

- 左归丸联合温和灸对骨质疏松症模型大鼠骨密度、骨保护素mRNA及核因子κB受体活化因子配体mRNA的影响
- Impact of Zuogui Wan （左归丸） Combined with Mild-warm Moxibustion on Bone Mineral Density,Osteoprotegerin mRNA and Nuclear Factor κB Receptor Activator Ligand mRNA of Osteoporosis Model Rats
- 中医杂志 2017年58卷第15期页码：1319-1322
- 作者机构：
  
  1. 浙江中医药大学附属杭州第一医院
  2. 浙江中医药大学
- 作者简介：
- 基金信息：
  
  浙江省中医药青年课题研究计划（2009YA001）;浙江省自然科学基金（LY16H270010）
- DOI：10.13288/j.11-2166/r.2017.15.015
  中图分类号： R-332;R285.5
- 纸质出版：2017
- 稿件说明：
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[1]钱康,范永升.左归丸联合温和灸对骨质疏松症模型大鼠骨密度、骨保护素mRNA及核因子κB受体活化因子配体mRNA的影响[J].中医杂志,2017,58(15):1319-1322.

QIAN Kang, FAN Yongsheng. Impact of Zuogui Wan （左归丸） Combined with Mild-warm Moxibustion on Bone Mineral Density,Osteoprotegerin mRNA and Nuclear Factor κB Receptor Activator Ligand mRNA of Osteoporosis Model Rats[J]. Journal of Traditional Chinese Medicine2017, 58(15): 1319-1322.
[1]钱康,范永升.左归丸联合温和灸对骨质疏松症模型大鼠骨密度、骨保护素mRNA及核因子κB受体活化因子配体mRNA的影响[J].中医杂志,2017,58(15):1319-1322. DOI： 10.13288/j.11-2166/r.2017.15.015.

QIAN Kang, FAN Yongsheng. Impact of Zuogui Wan （左归丸） Combined with Mild-warm Moxibustion on Bone Mineral Density,Osteoprotegerin mRNA and Nuclear Factor κB Receptor Activator Ligand mRNA of Osteoporosis Model Rats[J]. Journal of Traditional Chinese Medicine2017, 58(15): 1319-1322. DOI： 10.13288/j.11-2166/r.2017.15.015.

摘要

目的探讨左归丸联合温和灸治疗骨质疏松症的可能作用机制。方法 40只雌性SD大鼠随机分为空白组、模型组、左归丸组、左归丸+温和灸组

每组10只。模型组、左归丸组、左归丸+温和灸组采用手术完整摘除双侧卵巢法制造骨质疏松症模型。造模3个月后左归丸组予左归丸10 ml/（kg·d）灌胃

左归丸+温和灸组予左归丸10 ml/（kg·d）灌胃+长强穴艾灸治疗

空白组和模型组予生理盐水10 ml/（kg·d）灌胃。各组均干预6周。干预前后测定大鼠股骨近端及第2腰椎骨密度

干预后通过PCR法检测大鼠骨组织骨保护素（OPG）mRNA和核因子κB受体活化因子配体（RANKL）mRNA表达水平。结果与空白组比较

模型组股骨、腰椎骨密度

骨组织OPG mRNA表达及OPG/RANKL降低

RANKL mRNA表达上升（P<0.05或P<0.01）;与模型组比较

左归丸组、左归丸+温和灸组大鼠股骨、腰椎骨密度

骨组织OPG mRNA表达及OPG/RANKL均升高

RANKL mRNA表达降低（P<0.05或P<0.01）

且左归丸+温和灸组大鼠股骨、腰椎骨密度

骨组织OPG mRNA表达及OPG/RANKL均较左归丸组升高

RANKL mRNA表达较左归丸组降低（P<0.05）。结论左归丸联合温和灸能明显升高骨质疏松症大鼠的骨密度

上调OPG mRNA表达、下调RANKL mRNA表达可能是其作用机制之一。

Abstract

Objective To explore the possible mechanism of Zuogui Wan（左归丸） combined with mild-warm moxibustion in treating osteoporosis. Methods Forty female SD rats were randomized into blank group

model group

Zuogui Wan group and Zuogui Wan + mild-warm moxibustion group

10 rats in each group. Rats in the model group

Zuogui Wan group and Zuogui Wan + mild-warm moxibustion group were made osteoporosis models by completely removing bilateral ovaries. Three months after modeling

the Zuogui Wan group was given Zuogui Wan 10ml/（ kg·d） by gavage. The Zuogui Wan + mild-warm moxibustion group was given Zuogui Wan 10 ml/（ kg·d） by gavage and moxibustion at " Changqiang（ DU1） "point. The blank group and the model group were given normal saline 10 ml/（ kg·d） by gavage. All groups were treated for 6 weeks. Rats’ bone mineral density of proximal femur and the second lumbar vertebra were measured before and after treatment. After treatment

mRNA expression levels of rats bone tissue osteoprotegerin（ OPG） and nuclear factor κB receptor activator ligand（ RANKL） were detected by polymerase chain reaction（ PCR） method. Results Compared with the blank group

bone mineral density of femur and lumbar vertebra

bone tissue OPG mRNA expression

as well as OPG/RANKL in the model group decreased;while RANKL mRNA expression increased（ P < 0. 05 or P < 0. 01）. Compared with the model group

rats’ bone mineral density of femur and lumbar vertebra

bone tissue OPG mRNA expression

as well as OPG/RANKL in the Zuogui Wan group and the Zuogui Wan + mild-warm moxibustion group increased; while RANKL mRNA expression decreased（ P < 0. 05 or P < 0. 01）. Moreover

rats’ bone mineral density of femur and lumbar vertebra

bone tissue OPG mRNA expression

as well as OPG/RANKL in the Zuogui Wan + mild-warm moxibustion group were higher than those in the Zuogui Wan group; while RANKL mRNA expression was lower（ P < 0. 05）. Conclusion Zuogui Wan combined with mild-warm moxibustion might increase osteoporosis rats’ bone mineral density. Up-regulating OPG mRNA expression and down-regulating RANKL mRNA expression seem to be one of the mechanisms.

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