Objective To explore the possible mechanism of Jiyin Granule（济阴颗粒） in the treatment of menopause syndrome. Methods Ten rats were randomly selected out of 105 rats as the control group

and the rest 95 rats were used to build rat model of menopause syndrome by intraperitoneal injection of 4-vinylcyclohexene diepoxide solution. Sixty successful modeling rats were randomly divided into a model group

a western medicine control group

a Chinese medicine control group and Jiyin Granule low

middle and high dose groups

with 10 rats in each group.After modeling

the control group and the model group were given 10 ml/（ kg·d） of distilled water by gavage. The Jiyin Granule low

middle and high dose groups were given 3. 9

7. 8

and 15. 6 g/（ kg·d） of Jiyin Granule by gavage

respectively. The western medicine control group was given 0. 105 mg/（ g·d） of Estradiol Valerate Tablets by gavage. The Chinese medicine control group was given 10. 5 g/（kg·d） of Kunbao Pills（坤宝丸） by gavage. All of above treatment were given daily for 30 consecutive days. The expression of p53 protein in ovarian cells was detected by immunohistochemistry. The expression of apoptosis inducing factor（ AIF） and Endonuclease G（ Endo-G） mRNA in ovarian cells were detected by fluorescence RT-PCR. Results Compared with those in the blank control group

the expression of p53 protein and the expression of AIF and Endo-G mRNA in the ovarian cells in the model group were all increased（ P < 0. 01）

while the expression of p53 protein

AIF mRNA and Endo-G mRNA in the ovary cells were reduced in the groups of different doses of Jiyin Granule（ P < 0. 01）. The down-regulation of p53 protein in the Jiyin Granules low dose group was the most significant

while down-regulation of AIF mRNA in the high dose group was the most significant（ P < 0. 01）. Conclusion Jiyin Granule could inhibit the Caspase independent apoptosis pathway and inhibit the apoptosis of ovarian cells

which may be one of its mechanisms in the treatment of menopause syndrome.