扶正化瘀胶囊对心肌梗死后心肌纤维化大鼠细胞外基质代谢的影响

祁轶斐; 任学娇; 娄利霞; 吴爱明; 赵明镜; 赵一舟; 成文堃; 王婕; 朱珂; 陈萌; 张冬梅

doi:10.13288/j.11-2166/r.2018.07.017

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扶正化瘀胶囊对心肌梗死后心肌纤维化大鼠细胞外基质代谢的影响

实验研究 | 更新时间：2021-11-10

- 扶正化瘀胶囊对心肌梗死后心肌纤维化大鼠细胞外基质代谢的影响
- Effect of Fuzheng Huayu Capsule（扶正化瘀胶囊） on the Metabolism of Extracellular Matrix in Rats with Myocardial Fibrosis after Myocardial Infarction
- 中医杂志 2018年59卷第7期页码：607-611
- 作者机构：
  
  1. 北京中医药大学东直门医院
  2. 北京中医药大学基础医学院
- 作者简介：
- 基金信息：
  
  国家自然科学基金（81473662）
- DOI：10.13288/j.11-2166/r.2018.07.017
  中图分类号： R285.5
- 纸质出版：2018
- 稿件说明：
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[1]祁轶斐,任学娇,娄利霞,吴爱明,赵明镜,赵一舟,成文堃,王婕,朱珂,陈萌,张冬梅.扶正化瘀胶囊对心肌梗死后心肌纤维化大鼠细胞外基质代谢的影响[J].中医杂志,2018,59(07):607-611.

QI Yifei, REN Xuejiao, LOU Lixia, et al. Effect of Fuzheng Huayu Capsule（扶正化瘀胶囊） on the Metabolism of Extracellular Matrix in Rats with Myocardial Fibrosis after Myocardial Infarction[J]. Journal of Traditional Chinese Medicine2018, 59(7): 607-611.
[1]祁轶斐,任学娇,娄利霞,吴爱明,赵明镜,赵一舟,成文堃,王婕,朱珂,陈萌,张冬梅.扶正化瘀胶囊对心肌梗死后心肌纤维化大鼠细胞外基质代谢的影响[J].中医杂志,2018,59(07):607-611. DOI： 10.13288/j.11-2166/r.2018.07.017.

QI Yifei, REN Xuejiao, LOU Lixia, et al. Effect of Fuzheng Huayu Capsule（扶正化瘀胶囊） on the Metabolism of Extracellular Matrix in Rats with Myocardial Fibrosis after Myocardial Infarction[J]. Journal of Traditional Chinese Medicine2018, 59(7): 607-611. DOI： 10.13288/j.11-2166/r.2018.07.017.

摘要

目的探讨扶正化瘀胶囊治疗心肌梗死后心肌纤维化的可能作用机制。方法 24只大鼠随机分为模型组、扶正化瘀组、卡托普利组、假手术组

每组6只。除假手术组外

其余各组采用左冠状动脉前降支结扎术制备大鼠心肌梗死模型。造模后扶正化瘀组给予扶正化瘀胶囊0.4 g/（kg·d）灌胃

卡托普利组给予卡托普利2.25 mg/（kg·d）灌胃

假手术组和模型组均给予等体积的去离子水灌胃

各组均每日1次

连续8周。采用天狼星红染色检测心肌胶原分数（CVF）

免疫组化、实时荧光定量PCR法分别检测Ⅰ型胶原、基质金属蛋白酶2（MMP-2）、基质金属蛋白酶9（MMP-9）、基质金属蛋白酶抑制剂1（TIMP-1）、基质金属蛋白酶抑制剂2（TIMP-2）蛋白及mRNA表达。结果与假手术组比较

模型组大鼠CVF、MMP-2/TIMP-2及Ⅰ型胶原、MMP-2、MMP-9、TIMP-1、TIMP-2蛋白及mRNA表达均升高（P<0.05或P<0.01）。与模型组比较

扶正化瘀胶囊组、卡托普利组CVF、MMP-2/TIMP-2及Ⅰ型胶原、MMP-2、MMP-9、TIMP-1、TIMP-2蛋白及mRNA表达降低（P<0.05或P<0.01）。与卡托普利组比较

扶正化瘀组CVF、TIMP-1蛋白表达升高

TIMP-1 mRNA表达降低（P<0.05或P<0.01）。结论扶正化瘀胶囊能改善大鼠心肌梗死后心肌纤维化

其机制可能与调控MMP-2、MMP-9、TIMP-1、TIMP-2蛋白和mRNA表达及MMP-2/TIMP-2、MMP-9/TIMP-1有关。

Abstract

Objective To explore the possible mechanism of Fuzheng Huayu Capsule（扶正化瘀胶囊） in treating myocardial fibrosis after myocardial infarction. Methods Twenty-four rats were randomly divided into model group

Fuzheng Huayu group

captopril group and sham operation group

with 6 rats in each group. Except for the sham operation group

the other groups were treated with left anterior descending coronary artery ligation to prepare myocardial infarction model in rats. After the modeling

the Fuzheng Huayu group was given Fuzheng Huayu Capsule 0. 4 g/（kg·d） by gavage

the captopril group was given captopril 2. 25 mg/（kg·d） by gavage

and both the sham operation and model groups were given equal volume of deionized water by gavage. Each group was treated 1 times a day for8 weeks. Myocardial collagen volume fraction（CVF） was detected by Sirius red staining. The expression of type I collagen

matrix metalloproteinase 2（MMP-2）

matrix metalloproteinase 9（MMP-9）

tissue inhibitor of metallopeptidase 1（TIMP-1）

tissue inhibitor of metallopeptidase 2（TIMP-2） protein and mRNA were separately detected by immunohistochemistry and real-time fluorescence quantitative PCR. Results Compared with the sham operation group

the expression of CVF

MMP-2/TIMP-2 and type I collagen

MMP-2

MMP-9

TIMP-1

TIMP-2 protein and mRNA were increased in the model group（P < 0. 05 or P < 0. 01）. Compared with the model group

the expression of CVF

MMP-2/TIMP-2 and type I collagen

MMP-2

MMP-9

TIMP-1

TIMP-2 protein and mRNA were decreased in the Fuzheng Huayu group and captopril group（P < 0. 05 or P < 0. 01）. Compared with the captopril group

the expression of CVF and TIMP-1 protein was increased and the TIMP-1 mRNA expression was decreased in Fuzheng Huayu group（P < 0. 05 or P < 0. 01）. Conclusion Fuzheng Huayu Capsule could improve myocardial fibrosis after myocardial infarction in rats. Its mechanism may be related to the regulation of the expression of MMP-2

MMP-9

TIMP-1

TIMP-2 protein and mRNA and MMP-2/TIMP-2 and MMP-9/TIMP-1.

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