Objective To investigate the preventive effect of Jianpi Wenshen Fang（健脾温肾方） on the recurrence and metastasis of lung cancer and its possible mechanism. Methods NCI-H460-Luci cells were used to establish a nude mouse model after subcutaneous transplantation and were randomly divided into invigorating spleen and warming kidney group

chemotherapy group and control group

with 16 mice in each group. On the first postoperative day

the invigorating spleen and warming kidney group was given intragastric administration of Jianpi Wenshen Fang（1. 1 g/ml） 0. 3 ml/d

once a day for 13 weeks; the chemotherapy group was given paclitaxel 15 mg/kg. The rats were intraperitoneally injected once every 5 days totally for 3 times. The control group was given normal saline0. 3 ml/d orally once a day for 13 weeks. Until 13 weeks after operation

tumor metastasis of nude mice was observed by in vivo imaging once a week. The survival time of tumor-free metastasis in each group was analyzed. At the 6 th week after operation

miR-18 a-5 p

miR182-5 p

miR21

miR106 b-5 p and related target genes breast cancer susceptibility gene 1（BRCA1）

recombinant human phosphatase and tensin homology（PTEN）

P53 tumor protein（TP53）

epidermal growth factor receptor（EGFR） mRNA expression levels were detected by real-time fluorescence quantitative PCR. Results Compared with the control group

the expression of miR-18 a-5 p and miR182-5 p decreased and the expression of BRCA1 mRNA increased in the nude mice of the invigorating spleen and warming kidney group and the chemotherapy group（P < 0. 05 or P < 0. 01）; The expression of miR21 and miR106 b-5 p in tumors of nude mice in the chemotherapy group was also lower than that in control group（P < 0. 05 or P < 0. 01）. There was no significant difference in the expression of EGFR

PTEN and TP53 mRNA in the tumors of each group（P > 0. 05）. The difference in the survival time of tumor-free metastasis between the invigorating spleen and warming kidney group and the control group was statistically significant（P < 0. 05）

and there was no significant difference between the chemotherapy group and the invigorating spleen and warming kidney group（P > 0. 05）. Conclusion Jianpi Wenshen Fang has a certain role in preventing postoperative metastasis of lung cancer

and its mechanism may be related to regulating miRNAs and related target gene mRNA.