Objective To study the possible mechanism of electro-acupuncture therapy on Parkinson’ s disease（ PD）. Methods A total of 48 mice were randomly divided into blank group

mode group

electro-acupuncture group and Meddoba group

with 12 mice in each group. 1-Methyl-4-phenyl 1 2 3 6 tetrahydropyridine（ MPTP） was used to establish PD model mice by intraperitoneal injection in all groups except blank group. 0. 2 ml saline solution was given to the blank group. Meddoba suspension（ 50 mg/kg） was given to Meddoba group

once a day. Electroacupuncture stimulation with coordination between kidney and brain was used in electro-acupuncture group on"Shenshu"（ BL23）

"Baihui"（ GV20） and"Taixi"（ K13）

once a day

and 20 minutes each time with unilateral alternative treatment. The interventions in each group were all continuous for 4 weeks. The behaviors

amount of positive cells and expression of tyrosine hydroxylase（ TH）

glial fibrillary acidic protein（ GFAP）

Ionized calcium bindingadaptor molecule-1（ Iba-1） in substantia nigra of the mice were observed. Results The behaviors of mice in model group were significantly inferior to the blank group

while Meddoba group and electro-acupuncture group were better than model group. The amount of positive cells and expression of TH in substantia nigra was significantly reduced in the model group

while GFAP and Iba-1 were obviously increased（ P < 0. 05）. Compared with the model group

the amount of positive cells and expression of TH was significantly increased in electro-acupuncture group and meddoba group

while GFAP and Iba-1 were significantly reduced（ P < 0. 05）. The index in meddoba group and electro-acupuncture group had no significant difference（ P > 0. 05）. Conclusion Electro-acupuncture therapy with coordination between kidney and brain can inhibit the activity of astrocyte and microglia

and further potentiate the protection of dopaminergic neuron

which might be the mechanism of PD.