Objective To study the mechanism of Jinkui Shenqi Pills（ 金匮肾气丸） on kidney-yang deficiency.Methods A total of 60 male Wistar rats were randomly divided into 20 rats in a normal group and 40 in experimental group. Thirty-eight successfully modeling rats were randomly divided into traditional Chinese medicine group and model group

with 19 rats in each group. Jinkui Shenqi Pills 8. 44 g/（ kg·d） was instragastrically given to the traditional Chinese medicine group

and 3 ml normal saline was given to the normal group and the model group

once a day for 3 weeks consecutively. Femoral cancellous bone tissues of each group were obtained to extract and prepare the total protein. Two-dimensional gel electrophoresis（ 2-DE） technique was used to display the cancellous bone proteome map of each group. PDQuest 8. 0 software was used to analyze the differentially expressed protein spots in each group. The matrix-assisted laser desorption ionization time of flight mass spectrometry（ MALDI-TOF-MS） was used to initially identify differentially expressed protein spots. Results There were 552 discernible proteins spots in each rat femoral cancellous bone total protein 2-DE gel map of 3 groups. Compared with the normal group

the model group had 23 differentially expressed proteins. Compared with the model group

traditional Chinese medicine group had 16 differentially expressed proteins. After treatment with Jinkui Shenqi Pill

the expression of up-regulated phosphoglycerate kinase 1 and the down-regulated 14-3-3ζ protein

reticulocalbin 3

myosin promyosin isomer 2α-3 chain

myosin α-4 chain

myosin isoform α-1 chain

40 s ribosomal protein sa

galactoside agglutinin 1

annexin A1

GDP dissociation inhibition factor 1

type I collagen α-1 chain

LOC683667 protein

annexin A5

peroxy redox protein 2 and protein disulfide isomerase A3 all present reserve trend adjustment. Conclusion The regulation of Jinkui Shenqi Pills on kidney-yang deficiency probably concern with the structure of femoral cancellous bone tissues

material transport

energy metabolism

proliferation and apoptosis of cells

etc.