Objective To observe the effects and probable mechanism of Xiaoai Jiedu Decoction（消癌解毒方） in treating liver cancer rat. Methods A total of 40 male Sprague-Dawley rats were randomly divided into normal group

model group

Xiaoai Jiedu Decoction low and high dose groups

with 10 rats in each group. The rats in the low and high Xiaoai Jiedu Decoction dose groups and model group were treated with 0. 01% diethylnitrosamine（DEN） solution for free drinking to establish liver cancer models. The Xiaoai Jiedu Decoction low dose group and high dose group respectively received 10. 87 g/（kg·d） and 21. 74 g/（kg·d） Xiaoai Jiedu Decoction solution for gavage administration at the 4 th week while the model group received 2 ml/d normal saline

6 days a week for 12 weeks. Liver tissues of all rats were taken for morphological observation at the end of the 16 th week. The liver weight was measured

and the organ coefficient was calculated. The samples were labeled with protein tandem mass tag（TMT） peptide segment

identified by the mass spectrometry

and the bioinformatics was analyzed by KOBAS 3. 0 software.Results A total of 1151 protein spots were identified. Compared with the normal group

there were 234 proteins which differentially expressed in the model group

including 151 up-regulated proteins and 83 down-regulated proteins. Compared with the model group

there were 144 differentially expressed proteins in the Xiaoai Jiedu Decoction low-dose group

including 56 up-regulated proteins and 88 down-regulated proteins. Compared with the model group

there were 182 differentially expressed proteins in the Xiaoai Jiedu Decoction high-dose group

including 94 up-regulated proteins and 88 down-regulated proteins. These differentially expressed proteins in Xiaoai Jiedu Decoction highdose group mainly involved in metabolism

inflammation

immune regulation

heat shock

oxidative stress

cell cycle and apoptosis

cell growth and differentiation

angiogenesis

cancer-related and liver regeneration repair. Signaling pathway analysis involved metabolic pathways

such as glutathione metabolism

peroxisome proliferator-activated receptors（PPAR） metabolism

extracellular matrix receptor interaction（ECM-receptor-interaction） pathway

Toll-like receptors（TLRs） pathway

hypoxia-inducible factors-1（HIF-1） pathway

nuclear factor-κB（NF-κB）

mammalian target of rapamycin（m TOR） pathway

and p53 pathway. Conclusion Xiaoai Jiedu Decoction can play a multisystem

multi-target participation in the overall regulation of liver cancer model rats

regulation to inflammation-cancer transmisson may be the main mechanism of its inhibition of tumorigenesis and development.