Objective To evaluate the effects of formulae series for regulating and invigorating lung and kidney including Bufei Recipe（ 补肺方）

Bufei Jianpi Recipe（ 补肺健脾方）

Bufei Yishen Recipe（ 补肺益肾方）

Yiqi Zishen Recipe（ 益气滋肾方） on inflammation and oxidative stress in rats with lung injury induced by particulate matter（ PM）2. 5. Methods A total of 60 SD rats were randomly divided into blank group

model group

Bufei group

Bufei Jianpi group

Bufei Yishen group

Yiqi Zishen group. Real-time atmospheric concentrated PM 2. 5 were used to establish rats model for groups except the blank group. Since the first day exposed with PM 2. 5

rats in blank group and model group were gavaged with 0. 9% sodium chloride solution

2 ml for each rat; while rats in treatment groups were gavaged respectively with 5. 22 g/（ kg·d） Bufei Recipe

or 4. 84 g/（ kg·d） Bufei Jianpi Recipe

4. 44 g/（ kg·d）Bufei Yishen Recipe

4. 84 g/（ kg·d） Yiqi Zishen Recipe

one time per day. After continuous treatment for 14 days

the pulmonary function was detected

the histomorphological changes of lung tissue observed

serum levels of total antioxidant capacity（ T-AOC） and malondialdehyde（ MDA）

levels of tumor necrosis factor alpha（ TNF-α）

interleukin 6（ IL-6）

interleukin-1β（ IL-1β）

mucin 5 AC（ MUC5 AC） in alveolar lavage fluid

eosinophil major basic protein（ MBP）

neutrophil elastase（ ELA2）

IL-6

and IL-1β expression in lung tissue were detected. R-value comprehensive evaluation method was used to evaluate the comprehensive effect of each index. Results Compared with the blank group

pulmonary function in the model group was decreased. Airway and alveolar inflammatory infiltration increased in histomorphology. The levels of inflammatory cytokines（ TNF-α

IL-6

IL-1β

MBP

ELA2） in lung tissue all increased. T-AOC in serum decreased

whereas MDA increased（ P < 0. 05

P < 0. 01）. All of four methods for regulating and invigorating lung and kidney can improve the pulmonary function and pulmonary pathology condition

which could decrease the level of TNF-α

IL-6

IL-1β

MBP

ELA2 in the lung tissue

increase serum T-AOC

and reduce MDA（ P < 0. 05

P < 0. 01）. The R-value comprehensive evaluation results showed that the order of corrective effect intensity of all treatments on pulmonary function was Bufei Jianpi group→Bufei Yishen group→Yiqi Zishen→Bufei group→Bufei group; The order of corrective effect intensity on inflammation was Bufei Yishen group→Bufei Jianpi group → Bufei group → Yiqi Zishen group; The order of corrective effect intensity on oxidative stress was Bufei Yishen group→Bufei Jianpi group→Bufei group→Yiqi Zishen group; The order of comprehensive efficacy was Bufei Yishen group→Bufei Jianpi group→Bufei group→Yiqi Zishen group. Conclusion All of these four formulae for regulating and invigorating lung and kidney can comprehensively adjust alleviate inflammation and oxidative stress in lung injury induced by PM 2. 5

among which Bufei Yishen Recipe has significant advantages.